Human IL-17 ELISA Kit (Interleukin-17) (ab100556)

Overview

  • Product name
    Human IL-17 ELISA Kit (Interleukin-17)
    See all IL17 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    15.63 pg/ml - 1000 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 93.65 84% - 103%
    Plasma 92.24 82% - 104%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-17 (Interleukin-17) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-17 in plasma and cell culture supernatants.


    This assay employs an antibody specific for Human IL-17 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-17 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Human IL-17 in normal serum with ab100556, only in serum spiked with Human IL-17.


     

  • Notes

    Optimization may be required with urine samples

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    120X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human IL-17 2 vials
    IL-17 Microplate (12 x 8 wells) 1 unit
    Recombinant Human IL-17 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • Tissue specificity
    Restricted to activated memory T-cells.
  • Sequence similarities
    Belongs to the IL-17 family.
  • Post-translational
    modifications
    Found both in glycosylated and nonglycosylated forms.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • CTLA 8
    • CTLA-8
    • CTLA8
    • Cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL 17
    • IL 17A
    • IL-17
    • IL-17A
    • IL17_HUMAN
    • Il17a
    • Interleukin 17A
    • Interleukin-17A
    • Interleukin17
    • Interleukin17A
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100556

  • Representative standard curve using ab100556

Protocols

References

ab100556 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

This kit recognizes IL-17A and does not cross react with IL-17B, C, D, E, or F. Whether the kit will also recognize the IL-17A/F heterodimer has not been tested but some degree of cross reactivity is possible based on sequence similarity.

Gracias por contactarnos.


He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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