Human IL-17 ELISA Kit (Interleukin-17) (ab100556)

Overview

  • Product nameHuman IL-17 ELISA Kit (Interleukin-17)
    See all IL17 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    15.63 pg/ml - 1000 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 93.65 84% - 103%
    Serum 94.15 82% - 103%
    Plasma 92.24 82% - 104%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-17 (Interleukin-17) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-17 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human IL-17 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-17 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

     

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionInduces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • Tissue specificityRestricted to activated memory T-cells.
  • Sequence similaritiesBelongs to the IL-17 family.
  • Post-translational
    modifications
    Found both in glycosylated and nonglycosylated forms.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • CTLA 8
    • CTLA-8
    • CTLA8
    • Cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL 17
    • IL 17A
    • IL-17
    • IL-17A
    • IL17_HUMAN
    • Il17a
    • Interleukin 17A
    • Interleukin-17A
    • Interleukin17
    • Interleukin17A
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-17 ELISA Kit (Interleukin-17) images

  • Representative standard curve using ab100556

  • Representative standard curve using ab100556

Protocols

References for Human IL-17 ELISA Kit (Interleukin-17) (ab100556)

ab100556 has not yet been referenced specifically in any publications.

Product Wall

This kit recognizes IL-17A and does not cross react with IL-17B, C, D, E, or F. Whether the kit will also recognize the IL-17A/F heterodimer has not been tested but some degree of cross reactivity is possible based on sequence similarity.

Gracias por contactarnos.


He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas espec&iacu...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"