Overview

  • Product name
    Human IL-17a ELISA Kit (Interleukin -17a)
    See all IL-17a kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 6 3.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 6 5.2%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 3 pg/ml
  • Range
    3.12 pg/ml - 100 pg/ml
  • Recovery

    105 %

    Sample specific recovery
    Sample type Average % Range
    Serum 121 113% - 130%

  • Assay time
    3h 45m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-17a (Interleukin-17a) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative of IL-17a in supernatants, buffered solutions, serum, plasma and other body fluids.


    A monoclonal antibody specific for IL-17a has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-17a concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-17a are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-17a present in the samples.


    This kit will recognize both endogenous and recombinant Human IL-17a.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab83688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • IL-17 measured in cell culture supernatants (dilution range 1/1-1/100) from control (PBMC) or treated ((PBMC+PHA) 48 hours; 2% PHA-M; LifeTechnologies) cells (duplicates +/- SD).

  • Representative Standard Curve using ab83688

Protocols

References

This product has been referenced in:
  • Yu X  et al. Intensive Cytokine induction in Pandemic H1N1 Influenza Virus Infection Accompanied by Robust Production of IL-10 and IL-6. PLoS One 6:e28680 (2011). ELISA . Read more (PubMed: 22174866) »

See 1 Publication for this product

Customer reviews and Q&As

Gracias por contactarnos.


He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas espec&iacu...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up