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|Sample type||Average %||Range|
|Cell culture supernatant||75.78||68% - 85%|
|Serum||80.9||71% - 89%|
|Plasma||74.98||67% - 83%|
Abcam’s IL-18 R beta (Interleukin-18 R beta) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-18 R beta in serum, plasma, cell culture supernatants. (Human IL-18 R beta concentration is pretty low in normal serum/plasma, it may not be detected in this assay)
This assay employs an antibody specific for Human IL-18 R beta coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-18 R beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-18 R beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-18 R beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|500X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|5X Assay Diluent D||1 x 15ml|
|Biotinylated anti-Human IL-18 R beta||2 vials|
|IL-18 R beta Microplate||1 unit|
|Recombinant Human IL-18 R beta Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab113325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Representative Standard Curve using ab113325
ab113325 has not yet been referenced specifically in any publications.