Human IL-18 R beta ELISA Kit (Interleukin-18 R beta) (ab113325)

Overview

  • Product name
    Human IL-18 R beta ELISA Kit (Interleukin-18 R beta)
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 12 pg/ml
  • Range
    12.3 pg/ml - 3000 pg/ml
  • Recovery

    77 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 75.78 68% - 85%
    Serum 80.9 71% - 89%
    Plasma 74.98 67% - 83%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-18 R beta (Interleukin-18 R beta) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-18 R beta in serum, plasma, cell culture supernatants. (Human IL-18 R beta concentration is pretty low in normal serum/plasma, it may not be detected in this assay)

    This assay employs an antibody specific for Human IL-18 R beta coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-18 R beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-18 R beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-18 R beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    5X Assay Diluent D 1 x 15ml
    Biotinylated anti-Human IL-18 R beta 2 vials
    IL-18 R beta Microplate 1 unit
    Recombinant Human IL-18 R beta Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Required for the high affinity binding of interleukin 18 (IL-18) to its receptor complex (By similarity). Together with IL18R1 mediates IL-18-dependent activation of NF-kappa-B and JNK.
  • Tissue specificity
    Strongly expressed in peripheral blood leukocytes, spleen, lung, and, to a lesser extent, in colon, but not in any other tissue tested. Strongly expressed in T-cells polarized with IL-12. Specifically coexpressed with IL18R1 in Th1 cells.
  • Sequence similarities
    Belongs to the interleukin-1 receptor family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Alternative names
    • IL18RAP
    • Accessory protein like
    • Accessory protein-like
    • AcPL
    • CD218 antigen-like family member B
    • CD218b
    • CDw218b
    • I18RA_HUMAN
    • IL 18RAcP
    • IL 1R accessory protein like
    • IL 1R7
    • IL 1RAcPL
    • IL-18 receptor accessory protein
    • IL-18R-beta
    • IL-18RAcP
    • IL-18Rbeta
    • IL-1R accessory protein-like
    • IL-1R-7
    • IL-1R7
    • IL-1RAcPL
    • IL18RAP
    • IL18Rbeta
    • Interleukin 18 receptor accessory protein
    • Interleukin-1 receptor 7
    • Interleukin-18 receptor accessory protein
    • Interleukin-18 receptor accessory protein-like
    • Interleukin-18 receptor beta
    • Interleukin-18 receptor beta chain
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab113325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab113325

Protocols

References

ab113325 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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