For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Abcam’s IL-1a (Interleukin-1 alpha) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1a in cell culture supernatants, buffered solutions, serum, plasma and other body fluids. This assay will recognize both natural and recombinant Human IL-1a.
A monoclonal antibody specific for IL-1a has been coated to the wells of the microtiter strip plate provided. Samples, including standards of known IL-1a concentrations and unknown are pipetted into these wells. During incubation, binding of IL-1a to the capture antibodies is completed and then any excess unbound analyte is removed by washing. A biotinylated monoclonal antibody specific for IL-1a is then incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1 alpha present in the sample.
This kit will recognize both endogenous and recombinant Human IL-1a.
|Components||Identifier||1 x 96 tests||2 x 96 tests|
|10X Standard Diluent Buffer||Black||1 x 25ml||1 x 25ml|
|200X Wash Buffer||White||1 x 10ml||2 x 10ml|
|Biotinylated Antibody Diluent||Red||1 x 7ml||1 x 13ml|
|Biotinylated anti-IL1a||Red||1 x 0.4ml||2 x 0.4ml|
|Chromogen TMB Substrate Solution||1 x 11ml||1 x 24ml|
|HRP Diluent||Red||1 x 23ml||1 x 23ml|
|IL1a Control||Silver||2 vials||4 vials|
|IL-1a Microplate (12 x 8 well strips)||1 unit||2 units|
|IL-1a Standard (Lyophilized)||Yellow||2 vials||4 vials|
|Standard Diluent (Human Serum)||1 x 7ml||2 x 7ml|
|Stop Reagent||Black||1 x 11ml||2 x 11ml|
|Streptavidin-HRP||2 x 5µl||4 x 5µl|
Our Abpromise guarantee covers the use of ab46028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab46028 has not yet been referenced specifically in any publications.