Human IL-1RA ELISA Kit (Interleukin-1RA antagonist) (ab100565)

Overview

  • Product nameHuman IL-1RA ELISA Kit (Interleukin-1RA antagonist)
    See all IL1RA antagonist kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.1 ng/ml
  • Range
    0.124 ng/ml - 30 ng/ml
  • Recovery

    92 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 93.45 93% - 103%
    Tissue Extracts 91.92 82% - 102%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human IL-1RA ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-1RA in cell lysates and tissue lysates.

    This assay employs an antibody specific for Human IL-1RA coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1RA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-1RA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1RA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionInhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
  • Tissue specificityThe intracellular form of IL1RN is predominantly expressed in epithelial cells.
  • Involvement in diseaseGenetic variation in IL1RN is associated with susceptibility to microvascular complications of diabetes type 4 (MVCD4) [MIM:612628]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
    Defects in IL1RN are the cause of interleukin 1 receptor antagonist deficiency (DIRA) [MIM:612852]; also known as deficiency of interleukin 1 receptor antagonist. Autoinflammatory diseases manifest inflammation without evidence of infection, high-titer autoantibodies, or autoreactive T-cells. DIRA is a rare, autosomal recessive, genetic autoinflammatory disease that results in sterile multifocal osteomyelitis (bone inflammation in multiple places), periostitis (inflammation of the membrane surrounding the bones), and pustulosis (due to skin inflammation) from birth.
  • Cellular localizationCytoplasm and Secreted.
  • Information by UniProt
  • Alternative names
    • Anakinra
    • DIRA
    • ICIL 1RA
    • ICIL-1RA
    • IL 1ra
    • IL 1RN
    • IL-1ra
    • IL-1RN
    • IL1 inhibitor
    • IL1F3
    • IL1RA
    • IL1RA_HUMAN
    • IL1RN
    • Interleukin 1 receptor antagonist
    • Interleukin 1 receptor antagonist protein
    • Interleukin-1 receptor antagonist protein
    • Intracellular IL 1 receptor antagonist type II
    • Intracellular interleukin 1 receptor antagonist
    • IRAP
    • MGC10430
    • MVCD4
    • Type II interleukin 1 receptor antagonist
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-1RA ELISA Kit (Interleukin-1RA antagonist) images

  • IL-1Ra measured in RIPA lysates from control cells (-) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours (IL-1Ra per million cells; duplicates +/- SD). 5e6 cells were lysed in 0.1 mL, and tested in the dilution range of 1/1-1/40.  

  • IL-1Ra measured in culture supernatants from control (-) or activated PBMCs ((PHA) 48 hours with 2% PHA-M; LifeTechnologies) and in human biological fluids. Signals from plasma and serum were below level of detection (duplicates +/- SD).

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Representative standard curve using ab100565

Protocols

References for Human IL-1RA ELISA Kit (Interleukin-1RA antagonist) (ab100565)

ab100565 has not yet been referenced specifically in any publications.

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Thank you for your inquiry. Unfortunately we have not tested this kit for cross reactivity with rat. If it helps, the capture antibody used is monoclonal and the detection antibody is polyclonal. If you do wish to test...

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