Overview

  • Product name

    Human IL-2 ELISA Kit (Interleukin-2)


    See all IL2 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample 6 3.4%
    Inter-assay
    Sample n Mean SD CV%
    Sample 6 9.4%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    31.2 pg/ml - 1000 pg/ml
  • Recovery

    94 %

  • Assay time
    1h 50m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-2 (Interleukin-2) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2 in supernatants, buffered soloutions, serum and plasma samples.


    A monoclonal antibody specific for IL-2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2 present in the samples.


    This kit will recognize both endogenous and recombinant Human IL-2.


    Get results in 90 minutes with Human IL-2 ELISA Kit (ab174444) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Function
    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • Involvement in disease
    Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
  • Sequence similarities
    Belongs to the IL-2 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Aldesleukin
    • IL 2
    • IL-2
    • IL2
    • IL2_HUMAN
    • Interleukin 2
    • Interleukin-2
    • Interleukin2
    • Involved in regulation of T cell clonal expansion
    • Lymphokine
    • T cell growth factor
    • T-cell growth factor
    • TCGF
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab46033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-2 ELISA Kit (Interleukin-2)

images

  • Representative standard curve using ab46033

Protocols

References for

Human IL-2 ELISA Kit (Interleukin-2)

(ab46033)

ab46033 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab46033.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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