Human IL-2 ELISA Kit (Interleukin-2) High Sensitivity (ab46054)

Overview

  • Product nameHuman IL-2 ELISA Kit (Interleukin-2) High Sensitivity
    See all IL2 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5 4.2%
    Inter-assay
    Sample n Mean SD CV%
    Overall 5 9%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.97 pg/ml
  • Range
    1.87 pg/ml - 60 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95 % - %
    Serum 100 % - %

  • Assay time
    2h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human High Sensitivity IL-2 ELISA is to be used for the in-vitro quantitative determination interleukin-2 (IL-2) in Human sera, plasmas, buffered solutions or cell culture media.

    A monoclonal antibody specific for IL-2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2 present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-2.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionProduced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • Involvement in diseaseNote=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
  • Sequence similaritiesBelongs to the IL-2 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Aldesleukin
    • IL 2
    • IL-2
    • IL2
    • IL2_HUMAN
    • Interleukin 2
    • Interleukin-2
    • Interleukin2
    • Involved in regulation of T cell clonal expansion
    • Lymphokine
    • T cell growth factor
    • T-cell growth factor
    • TCGF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-2 ELISA Kit (Interleukin-2) High Sensitivity images

  • Representative standard curve using ab46054

Protocols

References for Human IL-2 ELISA Kit (Interleukin-2) High Sensitivity (ab46054)

ab46054 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"