Human IL-2 Receptor ELISA Kit (Interleukin-2 Receptor) (ab46036)

Overview

  • Product nameHuman IL-2 Receptor ELISA Kit (Interleukin-2 Receptor)
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.5%
    Inter-assay
    Sample n Mean SD CV%
    1 6 1594pg/ml 184 11.6%
    2 6 1528pg/ml 135 8.8%
    3 6 1705pg/ml 204 12%
    4 6 1124pg/ml 171 15.2%
    5 6 2305pg/ml 165 7.2%
    6 6 1824pg/ml 126 69%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    32.5 pg/ml
  • Range
    68.75 pg/ml - 2220 pg/ml
  • Recovery

    87 %

    Sample specific recovery
    Sample type Average % Range
    Serum 99 86% - 122%

  • Assay time
    3h 40m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-2 Receptor (IL-2R) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2R in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant Human IL-2R.

    A monoclonal antibody specific for IL-2R has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2R concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2R are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2R present in the samples. 

    This kit will recognize both endogenous and recombinant Human IL-2 Receptor.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceThe IL2 Receptor, which is involved in T cell-mediated immune responses, alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Homodimeric beta (IL2RB) chains produce a medium-affinity receptor. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The interleukin 2 (IL2) receptor gamma chain (IL2RG), an important signalling component of many interleukin receptors (IL2,IL4,IL7,IL9, and IL15), is thus referred to as the common gamma chain. Mutations in this X-chromosome-linked gene cause X-linked severe combined immunodeficiency (XSCID).
  • Cellular localizationCell Membrane; single-pass type I membrane protein.
  • Alternative names
    • CD122
    • CD122 antigen
    • CD132
    • CD132 antigen
    • CD25
    • CD25 antigen
    • IL2RA
    • IL2RB
    • IL2RG
    • IMD4
    • interleukin 2 receptor alpha
    • interleukin 2 receptor beta
    • interleukin 2 receptor gamma
    • p55
    • p64
    • p75
    • SCIDX
    • SCIDX1
    • TAC antigen
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-2 Receptor ELISA Kit (Interleukin-2 Receptor) images

  • Representative Standard Curve using ab46036

Protocols

References for Human IL-2 Receptor ELISA Kit (Interleukin-2 Receptor) (ab46036)

ab46036 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. The two soluble IL2 receptor ELISA kits, ab46036 (with plate) and ab47354 (capture and detection antibodies sufficient for five plates, but plates are not included) are known to be reactive with the alpha sub-unit. Reactivi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"