|Sample type||Average %||Range|
|Cell culture supernatant||95||% - %|
|Serum||71||% - %|
Abcam’s IL-21 (Interleukin-21) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human IL-21 (Interleukin-21)concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate).
IL-21 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated IL-21 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-21 captured on the plate.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||4 units|
|Biotin-Conjugate anti-human IL21 monoclonal antibody||1 x 70µl|
|Human IL-21 Standard lyophilized (10 ng/ml upon reconstitution)||2 vials|
|Microplate coated with monoclonal antibody to Human IL-21||1 unit|
|Sample Diluent||1 x 12ml|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|Streptavidin-HRP||1 x 150µl|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119542 has not yet been referenced specifically in any publications.