|Sample type||Average %||Range|
|Cell culture supernatant||123||% - %|
|Serum||92||% - %|
Abcam’s IL-22 (Interleukin-22) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-22 in cell culture supernatant, serum, and plasma (EDTA, citrate, heparin).
IL-22 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Human IL-22 detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-22 captured on the plate.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||6 units|
|Biotin-Conjugate anti-Human IL-22 monoclonal antibody||1 x 140µl|
|IL-22 Standard lyophilized (4 ng/mL upon reconstitution)||2 vials|
|Microplate coated with monoclonal antibody to IL-22 (12 x 8 wells)||1 unit|
|Sample Diluent||1 x 12ml|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|Streptavidin-HRP||1 x 150µl|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119543 has not yet been referenced specifically in any publications.