Human IL-27 ELISA Kit (ab83695)
Key features and details
- Sensitivity: 12.8 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-27 ELISA Kit
See all IL-27-A kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% A 9 343pg/ml 12.9 4.8% B 9 127pg/ml 8.1 5.1% C 9 427pg/ml 15.8 3.8% Inter-assay Sample n Mean SD CV% A 9 343pg/ml 29 8.5% B 9 157pg/ml 17 10.8% C 9 427pg/ml 38 9% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 12.8 pg/ml -
Range
31.25 pg/ml - 1000 pg/ml -
Recovery
95 %
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Assay time
3h 45m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s IL-27 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-27 in Human serum, plasma, buffered solutions and cell culture medium.
A monoclonal antibody specific IL-27 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-27 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-27 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-27 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-27-A and IL-27-B.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 2 x 96 tests 1 x 96 tests 1X Standard Diluent Buffer 2 x 25ml 1 x 25ml 200X Wash Buffer White 2 x 10ml 1 x 10ml Biotinylated anti IL-27 2 x 400µl 1 x 400µl Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7ml Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml HRP Diluent Red 1 x 23ml 1 x 12ml IL-27 Microplate (12 x 8 well strips) 2 units 1 unit IL-27 Standard (Lyophilized) 4 vials 2 vials Plastic Plate Covers 4 units 2 units Stop Reagent Black 2 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl -
Research areas
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Function
Cytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL-27 subunit p28 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replivation. -
Tissue specificity
Expressed in monocytes and in placenta. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Secreted. Does not seem to be secreted without coexpression of EBI3. - Information by UniProt
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Alternative names
- EBI3
- IL 27
- IL 27 p28 subunit
see all -
Database links
- Entrez Gene: 246778 Human
- Omim: 608273 Human
- SwissProt: Q8NEV9 Human
- Unigene: 528111 Human
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab83695 has been referenced in 2 publications.
- Abdel-Tawab MS et al. Evaluation of Serum and Gene Expression of Galectin-4, Interleukin-27, and Complement-7 in Hepatitis C Virus-Infected Egyptian Patients. Biomed Res Int 2020:8879758 (2020). PubMed: 33381596
- Cimato TR & Palka BA Effects of statins on TH1 modulating cytokines in human subjects. PeerJ 3:e764 (2015). PubMed: 25699211