Human IL-29 ELISA Kit (Interleukin-29) (ab100568)

Overview

  • Product nameHuman IL-29 ELISA Kit (Interleukin-29)
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    13.72 pg/ml - 10000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 124 104% - 143%
    Serum 117.1 105% - 132%
    Plasma 112.7 95% - 145%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-29 (Interleukin-29) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-29 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-29 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-29 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-29 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-29 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionCytokine with immunomodulatory activity. May play a role in antiviral immunity. Up-regulates MHC class I antigen expression. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway.
  • Sequence similaritiesBelongs to the IL-28/IL-29 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Cytokine ZCYTO21
    • IFN lambda 1
    • IFN-lambda-1
    • IFNL1
    • IL 29
    • IL-29
    • IL29
    • IL29_HUMAN
    • Interferon lambda 1
    • Interferon lambda-1
    • Interferon, lambda 1
    • Interleukin 29
    • Interleukin-29
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-29 ELISA Kit (Interleukin-29) images

  • Representative standard curve using ab100568

  • IL-29 measured in biological fluids, background signal subtracted (duplicates +/- SD).
  • Representative standard curve using ab100568

Protocols

References for Human IL-29 ELISA Kit (Interleukin-29) (ab100568)

ab100568 has not yet been referenced specifically in any publications.

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Capture antibody: 100% cross­reactivity with recombinant human (rh) IL­28B/IFN­λ3 and 20% with rhIL­28A. No cross­reactivity was observed with rhIFN­αA, rhIFN­αB2...

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The QC data for these lots is attached. Please let me know if you have any questions or if there is anything else th...

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