|Sample type||Average %||Range|
|Cell culture supernatant||115% - 123%|
|Serum||86% - 106%|
Abcam’s IL-31 (Interleukin-31) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-31 in cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
IL-31 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an Biotin-conjugated anti-Human IL-31 detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-31 captured on the plate.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||6 units|
|Biotin-Conjugate anti-human IL31 polyclonal antibody||1 x 140µl|
|Calibrator Diluent||1 x 5ml|
|Conjugate Diluent||1 x 15ml|
|IL-31 Standard lyophilized (500 pg U/mL upon reconstitution)||2 vials|
|Microplate coated with polyclonal antibody to Human IL-31 (12 x 8 wells)||1 unit|
|Sample Diluent||1 x 12ml|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|Streptavidin-HRP||1 x 150µl|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119546 has not yet been referenced specifically in any publications.