Human IL-31 ELISA Kit (Interleukin-31) (ab119546)

Overview

  • Product nameHuman IL-31 ELISA Kit (Interleukin-31)
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 3.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 2.5%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    1 pg/ml
  • Range
    7.8 pg/ml - 500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 115% - 123%
    Serum 86% - 106%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-31 (Interleukin-31) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-31 in cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

    IL-31 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an Biotin-conjugated anti-Human IL-31 detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-31 captured on the plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionActivates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity.
  • Tissue specificityDetected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • IL-31
    • IL31
    • IL31_HUMAN
    • Interleukin-31
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-31 ELISA Kit (Interleukin-31) images

  • Representative Standard Curve using ab119546.

Protocols

References for Human IL-31 ELISA Kit (Interleukin-31) (ab119546)

ab119546 has not yet been referenced specifically in any publications.

Product Wall

very good Elisa

Excellent Excellent 5/5 (Ease of Use)
Abreviews
We obtain reliable and reproducible results with this Elisa.
The protocol booklet is well explained, easy to understand and easy to perform.
I used this kit for serum samples from a comparison of healthy donor and atopic patients and and cell culture supernatants.
The kit is easy to use and quick compared to other Sandwich Elisa.
Username

Abcam user community

Verified customer

Submitted Apr 16 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"