Overview

  • Product nameHuman IL-33 ELISA Kit (Interleukin-33)
    See all IL33 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 6.9%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    0.2 pg/ml
  • Range
    7.8 pg/ml - 500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 73% - 92%
    Serum 67% - 79%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-33 (Interleukin-33) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-33 in cell culture supernatant, serum, and plasma (citrate, heparin).

    IL-33 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Human IL-33 detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-33 captured on the plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionCytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T helper type 2-associated cytokines.
  • Tissue specificityExpressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.
  • Sequence similaritiesBelongs to the IL-1 family. Highly divergent.
  • Post-translational
    modifications
    Proteolytically converted to a mature form by CASP1 in vitro and calpains in vivo.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • C9orf26
    • DVS27
    • DVS27 related protein
    • IL-1F11
    • IL-33
    • IL1F11
    • IL33
    • IL33_HUMAN
    • Interleukin-1 family member 11
    • Interleukin-33
    • NF-HEV
    • NFEHEV
    • Nuclear factor from high endothelial venules
    • RP11-575C20.2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-33 ELISA Kit (Interleukin-33) images

  • Representative Standard Curve using ab119547.

Protocols

References for Human IL-33 ELISA Kit (Interleukin-33) (ab119547)

ab119547 has not yet been referenced specifically in any publications.

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