|Sample type||Average %||Range|
|Cell culture supernatant||73% - 92%|
|Serum||67% - 79%|
Abcam’s IL-33 (Interleukin-33) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-33 in cell culture supernatant, serum, and plasma (citrate, heparin).
IL-33 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Human IL-33 detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-33 captured on the plate.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||6 units|
|Biotin-Conjugate anti-human IL33 polyclonal antibody||1 x 140µl|
|Calibrator Diluent||1 x 5ml|
|Conjugate Diluent||1 x 15ml|
|IL-33 Standard lyophilized (500 pg/mL upon reconstitution)||2 vials|
|Microplate coated with polyclonal antibody to IL-33 (12 x 8 wells)||1 unit|
|Sample Diluent||1 x 12ml|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|Streptavidin-HRP||1 x 150µl|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119547 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119547 has not yet been referenced specifically in any publications.