|Sample type||Average %||Range|
|Serum||100||75% - 121%|
Abcam’s IL-4 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-4 in supernatants, buffered solutions, serum and plasma samples.
A monoclonal antibody specific IL-4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL4 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-4.
|Components||Identifier||1 x 96 tests||2 x 96 tests|
|10X Standard Diluent Buffer||Black||1 x 25ml||1 x 25ml|
|200X Wash Buffer||White||1 x 10ml||2 x 10ml|
|Biotinylated anti IL-4||1 x 400µl||2 x 400µl|
|Biotinylated Antibody Diluent||Red||1 x 7ml||1 x 13ml|
|Chromogen TMB Substrate Solution||1 x 11ml||1 x 24ml|
|Control||2 vials||4 vials|
|HRP Diluent||Red||1 x 23ml||1 x 23ml|
|IL-4 Microplate (12 x 8 well strips)||1 unit||2 units|
|IL-4 Standard (Lyophilized)||2 vials||4 vials|
|Standard Diluent (Serum)||1 x 7ml||2 x 7ml|
|Stop Reagent||Black||1 x 11ml||2 x 11ml|
|Streptavidin-HRP||2 x 5µl||4 x 5µl|
Our Abpromise guarantee covers the use of ab46058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab46058 has not yet been referenced specifically in any publications.