Human IL-4 ELISA Kit (Interleukin-4) High Sensitivity (ab46063)

Overview

  • Product name
    Human IL-4 ELISA Kit (Interleukin-4) High Sensitivity
    See all IL4 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 8 7.43pg/ml 0.9 12.3%
    B 8 6.2pg/ml 0.7 10.8%
    C 8 8.77pg/ml 0.7 7.6%
    D 8 7.36pg/ml 0.4 6%
    Inter-assay
    Sample n Mean SD CV%
    A 9 7.1pg/ml 0.83 11.8%
    B 9 6.47pg/ml 0.72 11.1%
    C 9 8.76pg/ml 1.37 15.7%
    D 9 7.44pg/ml 1.2 16.1%
    E 7 2pg/ml 0.874 17%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 0.31 pg/ml
  • Range
    0.31 pg/ml - 10 pg/ml
  • Recovery

    101 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95 97% - 127%
    Serum 69 61% - 75%

  • Assay time
    4h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-4 (Interleukin-4) Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-4 in Human serum, plasma, buffered solution and cell culture media.

    A monoclonal antibody specific for IL-4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-4 present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-4.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml
    200X Wash Buffer White 1 x 10ml
    Amplification Diluent Brown & blue spot 1 x 25ml
    Amplifier Yellow 1 x 200µl
    Biotinylated anti IL-4 1 x 400µl
    Biotinylated Antibody Diluent Red 1 x 7.5ml
    Chromogen TMB Substrate Solution 1 x 11ml
    HRP Diluent Red 1 x 25ml
    IL-4 Microplate (12 x 8 well strips) 1 unit
    IL-4 Standard (Lyophilized) 2 vials
    Stop Reagent Black 1 x 11ml
    Streptavidin-HRP 2 x 5µl
  • Research areas
  • Relevance
    IL4 is a pleiotropic cytokine produced by activated T cells. It is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of IL4. IL4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of IL4 encoding distinct isoforms have been reported.
  • Cellular localization
    Secreted
  • Alternative names
    • B cell growth factor 1
    • B cell stimulatory factor 1
    • BCGF 1
    • BCGF1
    • Binetrakin
    • BSF 1
    • BSF1
    • IL 4
    • Interleukin 4
    • Lymphocyte stimulatory factor 1
    • MGC79402
    • Pitrakinra
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard curve of human IL-4, with background signal subtracted (duplicates; +/- SD).

  • IL-4 measured in cell culture supernatants from control (PBMC) or treated (PBMC+PHA; 2% PHA-M; LifeTechnologies) cells, grown for 48 hours (duplicates +/- SD). Samples tested neat - 1/10, control supernatant gave signals below level of detection.

  •  Representative Standard Curve using ab46063

Protocols

References

ab46063 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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