Human IL-4 ELISA Kit (Interleukin-4) High Sensitivity (ab46063)

Overview

  • Product nameHuman IL-4 ELISA Kit (Interleukin-4) High Sensitivity
    See all IL4 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 8 7.43pg/ml 0.9 12.3%
    B 8 6.2pg/ml 0.7 10.8%
    C 8 8.77pg/ml 0.7 7.6%
    D 8 7.36pg/ml 0.4 6%
    Inter-assay
    Sample n Mean SD CV%
    A 9 7.1pg/ml 0.83 11.8%
    B 9 6.47pg/ml 0.72 11.1%
    C 9 8.76pg/ml 1.37 15.7%
    D 9 7.44pg/ml 1.2 16.1%
    E 7 2pg/ml 0.874 17%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.31 pg/ml
  • Range
    0.31 pg/ml - 10 pg/ml
  • Recovery

    101 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95 97% - 127%
    Serum 69 61% - 75%

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-4 (Interleukin-4) Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-4 in Human serum, plasma, buffered solution and cell culture media.

    A monoclonal antibody specific for IL-4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-4 present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-4.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceIL4 is a pleiotropic cytokine produced by activated T cells. It is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of IL4. IL4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of IL4 encoding distinct isoforms have been reported.
  • Cellular localizationSecreted
  • Alternative names
    • B cell growth factor 1
    • B cell stimulatory factor 1
    • BCGF 1
    • BCGF1
    • Binetrakin
    • BSF 1
    • BSF1
    • IL 4
    • Interleukin 4
    • Lymphocyte stimulatory factor 1
    • MGC79402
    • Pitrakinra
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-4 ELISA Kit (Interleukin-4) High Sensitivity images

  • Standard curve of human IL-4, with background signal subtracted (duplicates; +/- SD).

  • IL-4 measured in cell culture supernatants from control (PBMC) or treated (PBMC+PHA; 2% PHA-M; LifeTechnologies) cells, grown for 48 hours (duplicates +/- SD). Samples tested neat - 1/10, control supernatant gave signals below level of detection.

  •  Representative Standard Curve using ab46063

Protocols

References for Human IL-4 ELISA Kit (Interleukin-4) High Sensitivity (ab46063)

ab46063 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab46063.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"