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|Sample type||Average %||Range|
|Cell culture supernatant||95||97% - 127%|
|Serum||69||61% - 75%|
Abcam’s IL-4 (Interleukin-4) Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-4 in Human serum, plasma, buffered solution and cell culture media.
A monoclonal antibody specific for IL-4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-4 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-4.
|Components||Identifier||1 x 96 tests|
|10X Standard Diluent Buffer||Black||1 x 25ml|
|200X Wash Buffer||White||1 x 10ml|
|Amplification Diluent||Brown & blue spot||1 x 25ml|
|Amplifier||Yellow||1 x 200µl|
|Biotinylated anti IL-4||1 x 400µl|
|Biotinylated Antibody Diluent||Red||1 x 7.5ml|
|Chromogen TMB Substrate Solution||1 x 11ml|
|HRP Diluent||Red||1 x 25ml|
|IL-4 Microplate (12 x 8 well strips)||1 unit|
|IL-4 Standard (Lyophilized)||2 vials|
|Stop Reagent||Black||1 x 11ml|
|Streptavidin-HRP||2 x 5µl|
Our Abpromise guarantee covers the use of ab46063 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Standard curve of human IL-4, with background signal subtracted (duplicates; +/- SD).
IL-4 measured in cell culture supernatants from control (PBMC) or treated (PBMC+PHA; 2% PHA-M; LifeTechnologies) cells, grown for 48 hours (duplicates +/- SD). Samples tested neat - 1/10, control supernatant gave signals below level of detection.
Representative Standard Curve using ab46063
ab46063 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"