Human IL-4 ELISPOT Kit (without plates) (ab46547)

Overview

• Product nameHuman IL-4 ELISPOT Kit (without plates)
  See all IL4 kits
• Sample type
  Suspension cells
• Assay typeSandwich (qualitative)
• Assay durationMultiple steps standard assay
• Species reactivity
  Reacts with: Human
  
• Product overview

  Abcam’s IL-4 Human ELISPOT kit is an in vitro ELISPOT assay designed for the qualitative measurement of IL-4 production and secretion in a single cell suspension.

   

  The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. ELISPOT assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

   

  After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.

  
  This ELISPOT assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

  
  Recognizes natural human IL4

• Tested applicationsSuitable for: ELISpotmore details
• PlatformReagents

Properties

• Storage instructionsStore at +4°C. Please refer to protocols.

• Components	5 x 96 tests	10 x 96 tests	15 x 96 tests	20 x 96 tests
  Bovine Serum albumin	1 x 1g	2 x 1g	3 x 1g	4 x 1g
  IL-4 Biotinylated detection antibody	1 x 0.55ml	2 x 0.55ml	3 x 0.55ml	4 x 0.55ml
  IL-4 Capture antibody	1 x 0.5ml	2 x 0.5ml	3 x 0.5ml	4 x 0.5ml
  Ready-to-use BCIP/NBT substrate buffer	1 x 50ml	2 x 50ml	3 x 50ml	4 x 50ml
  Streptavidin - Alkaline Phosphatase conjugated	1 x 50µl	2 x 50µl	3 x 50µl	4 x 50µl

• Research areas
  • Immunology
  • Innate Immunity
  • Cytokines
  • Interleukins

  • Cancer
  • Tumor immunology
  • Cytokines
  • Interleukins

  • Kits/ Lysates/ Other
  • Kits
  • ELISPOT and FLUOROSPOT Kits
  • ELISPOT Kits
  • Interleukin ELISPOT kits