Overview

  • Product name
    Human IL-4 ELISPOT Kit (without plates)
    See all IL4 kits
  • Sample type
    Suspension cells
  • Assay type
    Sandwich (qualitative)
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-4 Human ELISPOT kit is an in vitro ELISPOT assay designed for the qualitative measurement of IL-4 production and secretion in a single cell suspension.

     

    The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. ELISPOT assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

     

    After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.


    This ELISPOT assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.


    Recognizes natural human IL4

  • Tested applications
    Suitable for: ELISpotmore details
  • Platform
    Reagents

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 5 x 96 tests 10 x 96 tests 15 x 96 tests 20 x 96 tests
    Bovine Serum Albumin 1 x 1g 2 x 1g 3 x 1g 4 x 1g
    IL-4 Biotinylated detection antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml 4 x 0.55ml
    IL-4 Capture antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml 4 x 0.5ml
    Ready-to-use BCIP/NBT substrate buffer 1 x 50ml 2 x 50ml 3 x 50ml 4 x 50ml
    Streptavidin - Alkaline Phosphatase conjugated 1 x 50µl 2 x 50µl 3 x 50µl 4 x 50µl
  • Research areas
  • Relevance
    IL4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. IL4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
  • Cellular localization
    Secreted
  • Alternative names
    • B cell stimulatory factor 1
    • Binetrakin
    • BSF1
    • Interleukin 4
  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab46547 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISpot Use at an assay dependent dilution.

    References

    ab46547 has not yet been referenced specifically in any publications.

    Publishing research using ab46547? Please let us know so that we can cite the reference in this datasheet.

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