Overview

  • Product nameHuman IL-5 ELISA Kit (Interleukin-5)
    See all IL5 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    2.74 pg/ml - 2000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.83 84% - 103%
    Serum 95.46 83% - 103%
    Plasma 96.32 84% - 104%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-5 (Interleukin-5) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-5 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-5 coated on a 96-well plate. Standards and samples are pipette into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionFactor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
  • Sequence similaritiesBelongs to the IL-5 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • B cell differentiation factor I
    • B-cell differentiation factor I
    • Colony stimulating factor eosinophil
    • EDF
    • Eosinophil differentiation factor
    • IL 5
    • IL-5
    • IL5
    • IL5_HUMAN
    • Interleukin 5
    • Interleukin 5 (colony stimulating factor eosinophil)
    • Interleukin-5
    • Interleukin5
    • T cell replacing factor
    • T-cell replacing factor
    • TRF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-5 ELISA Kit (Interleukin-5) images

  • Representative standard curve using ab100571

  • Representative standard curve using ab100571

Protocols

References for Human IL-5 ELISA Kit (Interleukin-5) (ab100571)

ab100571 has not yet been referenced specifically in any publications.

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