Overview

  • Product name
    Human IL-5 ELISA Kit (Interleukin-5)
    See all IL5 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample A 24 178pg/ml 5.05 2.8%
    Sample B 24 36.7pg/ml 1.97 5.36%
    Inter-assay
    Sample n Mean SD CV%
    Sample A 10 139.8pg/ml 4.75 3.4%
    Sample B 10 33.6pg/ml 2.31 6.8%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    7.8 pg/ml - 250 pg/ml
  • Assay time
    3h 45m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-5 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-5 in Human serum, plasma, buffered solutions and supernatants.

    A monoclonal antibody specific for IL-5 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-5 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-5 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-5 present in the samples. 

    This kit will recognize both endogenous and recombinant Human IL-5.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated anti IL-5 Red 1 x 400µl 2 x 400µl
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    Control Silver 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-5 Microplate (12 x 8 well strips) 1 unit 2 units
    IL5 standard Yellow 2 vials 4 vials
    Standard Diluent (Human Serum) 1 x 7ml 2 x 7ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas
  • Function
    Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
  • Sequence similarities
    Belongs to the IL-5 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • B cell differentiation factor I
    • B-cell differentiation factor I
    • Colony stimulating factor eosinophil
    • EDF
    • Eosinophil differentiation factor
    • IL 5
    • IL-5
    • IL5
    • IL5_HUMAN
    • Interleukin 5
    • Interleukin 5 (colony stimulating factor eosinophil)
    • Interleukin-5
    • Interleukin5
    • T cell replacing factor
    • T-cell replacing factor
    • TRF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab46026

Protocols

References

ab46026 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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