Overview

  • Product nameHuman IL-6 ELISA Kit (Interleukin-6)
    See all IL6 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Pooled sampl 15 4.2%
    Inter-assay
    Sample n Mean SD CV%
    Sample 1 18 200pg/ml 16 8.1%
    Sample 2 18 77pg/ml 8 10.1%
    Sample 3 18 63pg/ml 6 8.6%
    Sample 4 18 103pg/ml 6 6%
    Sample 5 18 53pg/ml 3 5.5%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    6.25 pg/ml - 200 pg/ml
  • Recovery

    96 %

  • Assay time
    1h 45m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-6 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-6 in Human serum, plasma, buffered solutions or supernatants.


    A monoclonal antibody specific for IL-6 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 present in the samples.


    This kit will recognize both endogenous and recombinant Human IL-6.


    For higher sensitivity in only 90 minutes, we recommend Human IL-6 ELISA Kit (ab178013) from our SimpleStep ELISA® range

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionCytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in diseaseGenetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • B cell differentiation factor
    • B cell stimulatory factor 2
    • B-cell stimulatory factor 2
    • BSF 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Cytotoxic T cell differentiation factor
    • Hepatocyte stimulating fact
    • HGF
    • HPGF
    • HSF
    • Hybridoma growth factor
    • Hybridoma plasmacytoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL 6
    • IL-6
    • IL6
    • IL6 protein
    • IL6_HUMAN
    • Interferon beta 2
    • Interferon beta-2
    • Interleukin 6
    • Interleukin 6 (interferon beta 2)
    • Interleukin BSF 2
    • Interleukin-6
    • Interleukin6
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab46027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-6 ELISA Kit (Interleukin-6) images

  • Standard curve of human IL-6 with background signal subtracted (duplicates; +/- SD).

  • IL-6 measured in cell culture supernatants (diluted 1/50-1/1250) from control or treated (48 hours with 2% PHA-M; LifeTechnologies) PBMCs (duplicates +/- SD).

  • IL-6 detected in positive control (Pos CTRL; ab46027), and in supernatants (diluted 1/1-1/5) from control cells or cells stimulated for 24 hours with 50 nanogram x mL-1 of PMA (ab120297) with 1 microgram  x mL-1 of LPS (Sigma) for the last 6 hours (PMA/LPS).

  • Representative Standard Curve using ab46027

  • Dilution curves of human IL-6 (ab46027) and NIBSC standard (89/548). One ng of standard IL-6 corresponds to 87.2 (+/- 8.0) IU NIBSC standard. Background signal subtracted (duplicates; +/- SD).

Protocols

References for Human IL-6 ELISA Kit (Interleukin-6) (ab46027)

This product has been referenced in:
  • Sajid F & Bano S Pro inflammatory interleukins and thyroid function in Naswar (dipping tobacco) users: a case control study. BMC Endocr Disord 16:47 (2016). Sandwich ELISA ; Human . Read more (PubMed: 27515932) »
  • Al Batran R  et al. Evaluation of the effect of andrographolide on atherosclerotic rabbits induced by Porphyromonas gingivalis. Biomed Res Int 2014:724718 (2014). ELISA . Read more (PubMed: 25215291) »

See all 3 Publications for this product

Product Wall

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Your buffer should be fine if you can keep the pH to around 7.0 for protein staibility.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or inform...

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Thank you for your inquiry.
Do you have the catalogue numbers of the kits you're inquiring about? We have 7 different IL6 Human ELISA kits in the catalogue: ab46044, ab46042, ab46027, ab46041, ab100572, ab100573 and ab108867.
The IL6 High Sen...

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Thank you very much for contacting us with your questions.

The wells will come as 12 strips of 8 wells each, so you can remove as many strips as you need from the pouch immediately before use, then store the remainingstrips in the pouch with ...

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All of the kits mentioned (ab46027, ab100512, ab100640, ab108891) are suitable for use with citrate plasma.

I hope this information has been of help. If your require any further information please do not...

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Thank you for contacting us. I apologise for the delay.
Unfortunately any of these kits have been tested with human ascites. However, they should work as they are suitable for all body fluids.
The only problem I would suspect is the high conc...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"