Abcam’s IL-6 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-6 in Human serum, plasma, buffered solutions or supernatants.
A monoclonal antibody specific for IL-6 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-6.
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|Components||Identifier||1 x 96 tests||2 x 96 tests|
|10X Standard Diluent Buffer||Black||1 x 25ml||1 x 25ml|
|200X Wash Buffer||White||1 x 10ml||2 x 10ml|
|Adhesive plate seal||1 x 2 units||1 x 4 units|
|Biotinylated Antibody Diluent||Red||1 x 7.5ml||1 x 13ml|
|Biotinylated anti-IL-6||Red||1 x 0.4ml||2 x 0.4ml|
|Chromogen TMB Substrate Solution||1 x 11ml||1 x 24ml|
|HRP Diluent||Red||1 x 23ml||1 x 23ml|
|IL-6 Control||Silver||2 vials||4 vials|
|IL-6 Microplate (12 x 8 well strips)||1 unit||2 units|
|IL-6 Standard (Lyophilized)||Yellow||2 vials||4 vials|
|Standard Diluent (Human Serum)||1 x 7ml||2 x 7ml|
|Stop Reagent||Black||1 x 11ml||2 x 11ml|
|Streptavidin-HRP||2 x 5µl||4 x 5µl|
Our Abpromise guarantee covers the use of ab46027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Standard curve of human IL-6 with background signal subtracted (duplicates; +/- SD).
IL-6 measured in cell culture supernatants (diluted 1/50-1/1250) from control or treated (48 hours with 2% PHA-M; LifeTechnologies) PBMCs (duplicates +/- SD).
IL-6 detected in positive control (Pos CTRL; ab46027), and in supernatants (diluted 1/1-1/5) from control cells or cells stimulated for 24 hours with 50 nanogram x mL-1 of PMA (ab120297) with 1 microgram x mL-1 of LPS (Sigma) for the last 6 hours (PMA/LPS).
Representative Standard Curve using ab46027
Dilution curves of human IL-6 (ab46027) and NIBSC standard (89/548). One ng of standard IL-6 corresponds to 87.2 (+/- 8.0) IU NIBSC standard. Background signal subtracted (duplicates; +/- SD).