Sample | n | Mean | SD | CV% |
---|---|---|---|---|
Overall | 3% |
Sample | n | Mean | SD | CV% |
---|---|---|---|---|
Overall | 7.4% |
99
%
Abcam’s IL-6 (Interleukin-6) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-6 concentrations in Human cell culture supernatants, serum and plasma.
An IL-6 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-6 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-6 captured in plate.
Components | 1 x 96 tests |
---|---|
100X Streptavidin-Peroxidase Conjugate | 1 x 80µl |
10X Diluent N Concentrate | 1 x 30ml |
20X Wash Buffer Concentrate | 2 x 30ml |
70X Biotinylated Human IL-6 Antibody | 1 x 90µl |
Chromogen Substrate | 1 x 8ml |
IL-6 Microplate (12 x 8 well strips) | 1 unit |
IL-6 Standard | 1 vial |
Sealing Tapes | 3 units |
Stop Solution | 1 x 12ml |
Our Abpromise guarantee covers the use of ab108867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Sandwich ELISA | Use at an assay dependent dilution. |
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"