Human IL-6 ELISA Kit (Interleukin-6) High Sensitivity (ab46042)

Overview

  • Product nameHuman IL-6 ELISA Kit (Interleukin-6) High Sensitivity
    See all IL6 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.4%
    Inter-assay
    Sample n Mean SD CV%
    Sample 1 18 25pg/ml 3 12.2%
    Sample 2 18 12pg/ml 1 8.7%
    Sample 3 18 6pg/ml 1 13.7%
    Sample 4 18 41pg/ml 2 5.9%
    Sample 5 18 31pg/ml 1 4.9%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.8 pg/ml
  • Range
    1.56 pg/ml - 50 pg/ml
  • Recovery

    93 %

  • Assay time
    3h 45m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-6 (Interleukin-6) Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-6 in supernatants, buffered solutions, serum, plasma and other body fluids. This assay will recognise both natural and recombinant Human IL-6.


    A monoclonal antibody specific for IL-6 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 present in the samples. 


    This kit will recognize both endogenous and recombinant Human IL-6.


    Save time with a 90-minute ELISA protocol. Try Human IL-6 ELISA Kit (ab178013) from our SimpleStep ELISA® range.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionCytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in diseaseGenetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • B cell differentiation factor
    • B cell stimulatory factor 2
    • B-cell stimulatory factor 2
    • BSF 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Cytotoxic T cell differentiation factor
    • Hepatocyte stimulating fact
    • HGF
    • HPGF
    • HSF
    • Hybridoma growth factor
    • Hybridoma plasmacytoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL 6
    • IL-6
    • IL6
    • IL6 protein
    • IL6_HUMAN
    • Interferon beta 2
    • Interferon beta-2
    • Interleukin 6
    • Interleukin 6 (interferon beta 2)
    • Interleukin BSF 2
    • Interleukin-6
    • Interleukin6
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab46042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-6 ELISA Kit (Interleukin-6) High Sensitivity images

  • IL-6 was measured in cell culture medium from U937 control cells, or cells stimulated with LPS (1 �g x mL-1; 6 h) or PMA (10 ng x mL-1; 24 h) and LPS (background signal subtracted) (duplicates +/- SD).
  • Representative Standard Curve using ab46042

Protocols

References for Human IL-6 ELISA Kit (Interleukin-6) High Sensitivity (ab46042)

This product has been referenced in:
  • Guest J  et al. Changes in oxidative damage, inflammation and [NAD(H)] with age in cerebrospinal fluid. PLoS One 9:e85335 (2014). Human . Read more (PubMed: 24454842) »
  • Olsen NJ  et al. Methotrexate induces production of IL-1 and IL-6 in the monocytic cell line U937. Arthritis Res Ther 16:R17 (2014). Human . Read more (PubMed: 24444433) »

See all 5 Publications for this product

Product Wall

Senior Principal Scientist

Excellent Excellent 5/5 (Ease of Use)
Abreviews
The assay has adquate sensitivity for my application. My application was to evaluate IL-6 level of human blood after exposed to Foreign Materials (FM) observed in certain blood circulation medical devices. The high sensitivity of the assay is very critical for my application. The assay has good linearity, it is very helpful.

I also want to comment on the fast delivery of the product and easy of use for the product ordering on line.
Username

Dr. Charlene Yuan

Verified customer

Submitted Aug 21 2014

1. The Biotinylated Antibody is provided in liquid form, it is stable at 4°C
2. Standards and Controls are provided lyophilized and should be reconstituted either by the volume stated on the vial or in the Protocol Booklet. They should be used ...

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Abreview for IL6 High Sensitivity Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Cell (Monocytes)
Specification: Monocytes
Amount of Sample: 10 µg
Type of Kit Used: ELISA
Did you use the Abcam Kit protocol?: Yes
Username

Abcam user community

Verified customer

Submitted Apr 29 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your message and for providing this further information.

I have reviewed this enquiry and discussed it with the originator of this kit. Regrettably, it does seem the second kit is not providing the sensitive results you require...

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Thank you for taking the time to contact us. Your message has been forwarded to the scientific support team. I am sorry to hear you have had difficulty obtaining satisfactory results from the second kit you received.

The details you have kin...

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Thank you for your inquiry.
Do you have the catalogue numbers of the kits you're inquiring about? We have 7 different IL6 Human ELISA kits in the catalogue: ab46044, ab46042, ab46027, ab46041, ab100572, ab100573 and ab108867.
The IL6 High Sen...

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Thank you for contacting us.

The lab let me know the following:
Saliva is suitable for thisELISA kit, and the regular protocol would apply. There is no need to pre-treat the samples before assaying.

I hope this information is...

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Thank you for confirming those details.

I have noticed that the results that you provided me with, there is a difference between the positive and negative samples by eye. How has your spectrophotometer been set up to measure this? At what wav...

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Thank you for your information.

I am sorry for the delay in getting back to you. I think it would be very helpful for us to understand exactly how the standard samples and streptavidin HRP was prepared.

As you say, this kit has been...

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1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"