Abcam’s IL-6 (Interleukin-6) Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-6 in supernatants, buffered solutions, serum, plasma and other body fluids. This assay will recognise both natural and recombinant Human IL-6.
A monoclonal antibody specific for IL-6 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-6.
Get results in 90 minutes with Human IL-6 ELISA Kit (ab178013) from our SimpleStep ELISA® range.
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
Involvement in disease
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Sandwich ELISA - IL-6 (Interleukin-6) High Sensitivity Human ELISA Kit (ab46042)
IL-6 was measured in cell culture medium from U937 control cells, or cells stimulated with LPS (1 �g x mL-1; 6 h) or PMA (10 ng x mL-1; 24 h) and LPS (background signal subtracted) (duplicates +/- SD).
Zhao C et al. Long-term bicycle riding ameliorates the depression of the patients undergoing hemodialysis by affecting the levels of interleukin-6 and interleukin-18. Neuropsychiatr Dis Treat13:91-100 (2017).
Read more (PubMed: 28096677) »
Sun L et al. Actinidia chinensis Planch. Improves the Indices of Antioxidant and Anti-Inflammation Status of Type 2 Diabetes Mellitus by Activating Keap1 and Nrf2 via the Upregulation of MicroRNA-424. Oxid Med Cell Longev2017:7038789 (2017).
Read more (PubMed: 28642811) »