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|Sample type||Average %||Range|
|Urine||93||92% - 94%|
|Serum||103||98% - 112%|
|Cell culture media||98||96% - 101%|
|Heparin Plasma||97||94% - 101%|
|EDTA Plasma||91||82% - 99%|
|Citrate Plasma||98||94% - 104%|
Abcam’s IL-6 Receptor (Interleukin-6 Receptor) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-6 Receptor (Interleukin-6 Receptor) protein in Human serum, plasma and cell culture supernatant.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Interleukin-6 Receptor (IL-6R, IL-6 Receptor), also known as Interleukin-6 Receptor subunit alpha and membrane glycoprotein 80, is part of a receptor that binds Interleukin-6 (IL-6). IL-6 Receptor weakly binds IL-6 with a low affinity, and then is presented to gp130 to facilitate transition into a high affinity signaling competent hexamer. Activation may lead to regulation of the immune response, acute-phase reactions and hematopoiesis. The production of IL-6 Receptor has been implicated in the pathogenesis of many diseases, including multiple myeloma, auto immune diseases and prostate cancer.IL-6 Receptor has two isoforms. The first is a transmembrane protein in which the N-terminal extracellular portion can be released by proteolytic cleavage. The second isoform is a secreted form that lacks the transmembrane and intracellular portions. This assay is directed at the extracellular portion of IL-6 Receptor and detects both the soluble and membrane bound forms.
|Components||1 x 96 tests|
|10X IL-6 Receptor Capture Antibody||1 x 600µl|
|10X IL-6 Receptor Detector Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|Antibody Diluent 4BI||1 x 6ml|
|IL-6 Receptor Human Lyophilized Recombinant Protein||2 vials|
|Plate Seals||1 unit|
|Sample Diluent NS (ab193972)||1 x 50ml|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 x 96 tests|
|Stop Solution||1 x 12ml|
|TMB Substrate||1 x 12ml|
Our Abpromise guarantee covers the use of ab181424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of IL-6 Receptor were interpolated from the IL-6 Receptor standard curve and corrected for sample dilution.
The concentrations of IL-6 Receptor were interpolated the IL-6 Receptor standard curve and corrected for sample dilution. The mean IL-6 Receptor concentration was determined to be 27,796 pg/mL in plasma (EDTA) and 25,731 pg/mL in serum.
Serum from 10 apparently healthy male donors was measured in duplicate for IL-6 Receptor. The mean IL-6 Receptor concentration was determined to be 24,806 pg/mL with a range of 17,960 – 35,807 pg/mL.
ab181424 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"