Human IL-6 Receptor ELISA Kit (Interleukin-6 Receptor) (ab46029)

Overview

  • Product nameHuman IL-6 Receptor ELISA Kit (Interleukin-6 Receptor)
    See all IL6R kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample A 7 977.7pg/ml 3.04 0.31%
    Sample B 7 131.1pg/ml 2.489 2.289%
    Inter-assay
    Sample n Mean SD CV%
    Sample A 24 983.9pg/ml 10.46 1.06%
    Sample B 24 136.2pg/ml 6.086 4.46%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    31.25 pg/ml - 1000 pg/ml
  • Recovery

    96 %

  • Assay time
    1h 40m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-6 Receptor (Interleukin-6 Receptor) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble IL-6 Receptor in Human serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific for IL-6 Receptor has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 Receptor concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 Receptor are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 Receptor present in the samples. 

    This kit will recognize both endogenous and recombinant Human IL-6 Receptor.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionPart of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
    Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.
  • Tissue specificityIsoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • DomainThe two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
  • Post-translational
    modifications
    A short soluble form may also be released from the membrane by proteolysis.
  • Cellular localizationSecreted and Basolateral cell membrane.
  • Information by UniProt
  • Alternative names
    • CD126
    • CD126 antigen
    • Gp80
    • IL 6R alpha
    • IL-6 receptor subunit alpha
    • IL-6R 1
    • IL-6R subunit alpha
    • IL-6R-1
    • IL-6R-alpha
    • IL-6RA
    • Il6r
    • IL6R1
    • IL6RA
    • IL6RA_HUMAN
    • IL6Ralpha
    • Interleukin 6 receptor
    • Interleukin-6 receptor subunit alpha
    • Membrane glycoprotein 80
    • MGC104991
    • OTTHUMP00000034320
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab46029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-6 Receptor ELISA Kit (Interleukin-6 Receptor) images

  • Representative Standard Curve using ab46029

Protocols

References for Human IL-6 Receptor ELISA Kit (Interleukin-6 Receptor) (ab46029)

ab46029 has not yet been referenced specifically in any publications.

Product Wall

Abreview for IL6R Human ELISA Kit

Good Good 4/5 (Ease of Use)
Abreviews

Sample: Human Cell (lung cancer cell line)
Specification: lung cancer cell line
Amount of Sample: about 1,000,000 cells µg
Type of Kit Used: ELISA
Positive control: The control vials in the kit (430.9 pg/mL)
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Optimized incubation time of TMB substrate solution was 10 minutes in our laboratory. With15 minutes Incubation, the O.D. value of 1000 pg/mL standard reached over 2.5,
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Mrs. Hee Yi

Verified customer

Submitted May 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"