Overview

  • Product name
    Human IL-7 ELISA Kit (Interleukin-7)
    See all IL7 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    4.1 pg/ml - 1000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.41 83% - 106%
    Serum 95.63 84% - 106%
    Plasma 96.37 85% - 108%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-7 (Interleukin-7) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-7 in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human IL-7 coated on a 96-well plate. Standards and samples are pipette into the wells and IL-7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Human IL-7 ELISA Kit (ab185984) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    160X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human IL-7 (lyophilized) 2 vials
    IL-7 Microplate (12 x 8 wells) 1 unit
    Recombinant Human IL-7 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    IL7 is a cytokine important for B and T cell development. It and the hepatocyte growth factor form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL7 is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. It can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that IL7 plays an essential role in lymphoid cell survival.
  • Cellular localization
    Secreted
  • Alternative names
    • IL 7
    • IL7 nirs variant 6
    • Interleukin 7
    • Interleukin7
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab100574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard curve (Diluent A) with background signal subtracted (duplicates; +/- SD).

  • Representative standard curve using ab100574

  • Human IL-7 measured in undiluted biological fluids (duplicates +/- SD).

  • Representative standard curve using ab100574

Protocols

References

ab100574 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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