• Product nameHuman IL-7 ELISA Kit (Interleukin-7)
    See all IL7 kits
  • Detection methodColorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    4.1 pg/ml - 1000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.41 83% - 106%
    Serum 95.63 84% - 106%
    Plasma 96.37 85% - 108%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-7 (Interleukin-7) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-7 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-7 coated on a 96-well plate. Standards and samples are pipette into the wells and IL-7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    Get higher sensitivity in only 90 minutes with Human IL-7 ELISA Kit (ab185984) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • RelevanceIL7 is a cytokine important for B and T cell development. It and the hepatocyte growth factor form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL7 is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. It can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that IL7 plays an essential role in lymphoid cell survival.
  • Cellular localizationSecreted
  • Alternative names
    • IL 7
    • IL7 nirs variant 6
    • Interleukin 7
    • Interleukin7
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab100574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-7 ELISA Kit (Interleukin-7) images

  • Standard curve (Diluent A) with background signal subtracted (duplicates; +/- SD).

  • Representative standard curve using ab100574

  • Human IL-7 measured in undiluted biological fluids (duplicates +/- SD).

  • Representative standard curve using ab100574


References for Human IL-7 ELISA Kit (Interleukin-7) (ab100574)

ab100574 has not yet been referenced specifically in any publications.

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