• Product nameHuman IL-7 ELISA Kit (Interleukin-7)
    See all IL7 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    4.1 pg/ml - 1000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.41 83% - 106%
    Serum 95.63 84% - 106%
    Plasma 96.37 85% - 108%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-7 (Interleukin-7) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-7 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-7 coated on a 96-well plate. Standards and samples are pipette into the wells and IL-7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • RelevanceIL7 is a cytokine important for B and T cell development. It and the hepatocyte growth factor form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL7 is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. It can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that IL7 plays an essential role in lymphoid cell survival.
  • Cellular localizationSecreted
  • Alternative names
    • IL 7
    • IL7 nirs variant 6
    • Interleukin 7
    • Interleukin7
  • Database links


Our Abpromise guarantee covers the use of ab100574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-7 ELISA Kit (Interleukin-7) images

  • Standard curve (Diluent A) with background signal subtracted (duplicates; +/- SD).

  • Representative standard curve using ab100574

  • Human IL-7 measured in undiluted biological fluids (duplicates +/- SD).

  • Representative standard curve using ab100574


References for Human IL-7 ELISA Kit (Interleukin-7) (ab100574)

ab100574 has not yet been referenced specifically in any publications.

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