Human IL-7 ELISA Kit (Interleukin-7) (ab46031)


  • Product nameHuman IL-7 ELISA Kit (Interleukin-7)
    See all IL7 kits
  • Detection methodColorimetric
  • Precision
    Sample n Mean SD CV%
    Sample A 8 198.7pg/ml 1.667 0.83%
    Sample B 8 28.69pg/ml 1.118 3.86%
    Sample n Mean SD CV%
    Sample A 29 198.9pg/ml 3.772 1.89%
    Sample B 29 25.68pg/ml 1.505 5.84%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 3 pg/ml
  • Range
    6 pg/ml - 200 pg/ml
  • Recovery

    97.6 %

  • Assay time
    2h 40m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-7 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-7 in Human serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific for IL-7 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-7 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-7 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-7 present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-7.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • RelevanceIL7 is a cytokine important for B and T cell development. It and the hepatocyte growth factor form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL7 is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. It can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that IL7 plays an essential role in lymphoid cell survival.
  • Cellular localizationSecreted
  • Alternative names
    • IL 7
    • IL7 nirs variant 6
    • Interleukin 7
    • Interleukin7
  • Database links


Our Abpromise guarantee covers the use of ab46031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-7 ELISA Kit (Interleukin-7) images

  • Representative Standard Curve using ab46031


References for Human IL-7 ELISA Kit (Interleukin-7) (ab46031)

ab46031 has not yet been referenced specifically in any publications.

Product Wall

Regrettably, the kit has not been validated with NIBSC IL-7 90/530. No calibration factor is available.

Sorry we could not be of further assistance in this instance. Please contact me again if you have any further questions.