Overview

  • Product name
    Human IL-8 ELISA Kit (Interleukin 8)
    See all IL8 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Pooled Data 3.1%
    Inter-assay
    Sample n Mean SD CV%
    Sample 1 18 1682pg/ml 152 9%
    Sample 2 18 1004pg/ml 111 11%
    Sample 3 18 768pg/ml 69 8.9%
    Sample 4 18 1497pg/ml 149 9.9%
    Sample 5 18 880pg/ml 83 9.4%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 25 pg/ml
  • Range
    62.5 pg/ml - 2000 pg/ml
  • Recovery

    88 %

  • Assay time
    1h 40m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-8 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-8 supernatants, buffered solutions, serum and plasma samples. (Genecards: CXCL8)


    A monoclonal antibody specific for IL-8 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-8 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-8 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-8 present in the samples.


    This kit will recognize both endogenous and recombinant Human IL-8.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Function
    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • (Ala-IL-8)77
    • (Ser-IL-8)72
    • 3-10C
    • AMCF-I
    • B-ENAP
    • Beta-thromboglobulin-like protein
    • C X C motif chemokine 8
    • C-X-C motif chemokine 8
    • Chemokine (C X C motif) ligand 8
    • CXCL8
    • Emoctakin
    • GCP 1
    • GCP-1
    • GCP/IL-8 protein I
    • GCP/IL-8 protein II
    • GCP/IL-8 protein III
    • GCP/IL-8 protein IV
    • GCP/IL-8 protein V
    • GCP/IL-8 protein VI
    • GCP1
    • Granulocyte chemotactic protein 1
    • IL 8
    • IL-8
    • IL-8(1-77)
    • IL-8(9-77)
    • IL8
    • IL8/NAP1 form I
    • IL8/NAP1 form II
    • IL8/NAP1 form III
    • IL8/NAP1 form IV
    • IL8/NAP1 form V
    • IL8/NAP1 form VI
    • IL8_HUMAN
    • Interleukin 8
    • K60
    • LECT
    • LUCT
    • Lymphocyte derived neutrophil activating factor
    • Lymphocyte-derived neutrophil-activating factor
    • LYNAP
    • MDNCF
    • MDNCF-b
    • MDNCF-c
    • MONAP
    • Monocyte derived neutrophil activating peptide
    • Monocyte derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil-activating peptide
    • NAF
    • NAP 1
    • NAP-1
    • NAP1
    • Neutrophil activating peptide 1
    • Neutrophil activating protein 1
    • Neutrophil-activating factor
    • Neutrophil-activating protein 1
    • Protein 3 10C
    • Protein 3-10C
    • SCYB8
    • Small inducible cytokine subfamily B member 8
    • T cell chemotactic factor
    • T-cell chemotactic factor
    • TSG-1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-8 ELISA Kit (Interleukin 8) images

  • Representative Standard Curve using ab46032

  • IL-8 detected in supernatants from control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), and with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours. The positive control is included (Pos.). Results shown after background signal was subtracted (duplicates +/- SD).

Protocols

References for Human IL-8 ELISA Kit (Interleukin 8) (ab46032)

This product has been referenced in:
  • Zhao C  et al. Long-term bicycle riding ameliorates the depression of the patients undergoing hemodialysis by affecting the levels of interleukin-6 and interleukin-18. Neuropsychiatr Dis Treat 13:91-100 (2017). ELISA ; Human . Read more (PubMed: 28096677) »
  • Prieto P  et al. Cell Expansion-Dependent Inflammatory and Metabolic Profile of Human Bone Marrow Mesenchymal Stem Cells. Front Physiol 7:548 (2016). ELISA ; Human . Read more (PubMed: 27899899) »

See all 10 Publications for this product

Product Wall

Nous ne recommandons pas de modifier les concentrations des courbes standards mais plutôt de diluer vos échantillons.

Toutefois, si vous voulez vraiment changer les courbes standards ceci est possible. Cependant en augmentant les ...

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Thank you very much for your call yesterday and for your patience while I have been in touch with the lab about ab46032.

This kit is suitable for all body fluids. Urine samples should be diluted in standard diluent buffer (containing PBS BSA)...

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Thank you for contacting us. I apologise for the delay.
Unfortunately any of these kits have been tested with human ascites. However, they should work as they are suitable for all body fluids.
The only problem I would suspect is the high conc...

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Thank you for your phone call yesterday and for your questions about our ELISA kits. I have heard back from the lab regarding ab46032, and unfortunately, the kit was never assayed on murine samples. So, I would not recommend this kit to use with mo...

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Thank you for your enquiry. There's no Thimerosal in any component of the kit. However, we use azide (0.1%) in standard diluent and Biotinylated antibody diluent, and Proclin (0.1%) in HRP diluent.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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