• Product nameHuman IL-9 ELISA Kit (Interleukin-9)
    See all IL9 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.1 ng/ml
  • Range
    0.04 ng/ml - 30 ng/ml
  • Recovery

    98 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 102.1 90% - 110%
    Serum 101.6 95% - 110%
    Plasma 82.4 67% - 98%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-9 (Interleukin-9) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-9 in serum, plasma and cell culture supernatants. (Human IL-9 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human IL-9 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • FunctionSupports IL-2 independent and IL-4 independent growth of helper T-cells.
  • Sequence similaritiesBelongs to the IL-7/IL-9 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Cytokine P40
    • Homolog of mouse T cell and mast cell growth factor 40
    • HP40
    • IL-9
    • Il9
    • IL9_HUMAN
    • Interleukin 9
    • Interleukin-9
    • Mast cell growth factor
    • MCGF
    • Megakaryoblast growth factor
    • p40 cytokine
    • T cell growth factor 3
    • T cell growth factor p40
    • T-cell growth factor P40
    • TCGF 3
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-9 ELISA Kit (Interleukin-9) images

  • Representative standard curve using ab100577

  • Representative standard curve using ab100577


References for Human IL-9 ELISA Kit (Interleukin-9) (ab100577)

ab100577 has not yet been referenced specifically in any publications.

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