Overview

  • Product name
    Human IL-9 ELISA Kit (Interleukin-9)
    See all IL9 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 0.1 ng/ml
  • Range
    0.04 ng/ml - 30 ng/ml
  • Recovery

    98 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 102.1 90% - 110%
    Plasma 82.4 67% - 98%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-9 (Interleukin-9) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-9 in plasma and cell culture supernatants. (Human IL-9 concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for Human IL-9 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Human IL-9 in normal serum with ab100577, only in serum spiked with Human IL-9.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100577

  • Representative standard curve using ab100577

Protocols

References

ab100577 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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