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Abcam’s IL-1α Human in vitro ELISA Set is designed for the quantitative measurement of IL-1α in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
This ELISA set is intended to measure human IL-1 alpha in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
The IL1 alpha ELISA set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
Recognizes both natural and recombinant human IL1a.
|Components||5 x 96 tests||10 x 96 tests||15 x 96 tests|
|Detection biotinylated anti-IL-1 alpha antibody||1 x 0.55ml||2 x 0.55ml||3 x 0.55ml|
|IL-1 alpha Capture antibody||1 x 0.1ml||2 x 0.1ml||3 x 0.1ml|
|IL1 alpha standard||5 vials||10 vials||15 vials|
|Ready-to-use TMB||2 vials||4 vials||6 vials|
|Streptavidin-HRP||1 vial||2 vials||3 vials|
Our Abpromise guarantee covers the use of ab47350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab47350 has not yet been referenced specifically in any publications.