Overview

  • Product nameHuman IL1 alpha ELISA Set (without plates)
  • Tests
    5 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    31.2 pg/ml - 1000 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-1α Human in vitro ELISA Set is designed for the quantitative measurement of IL-1α in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    This ELISA set is intended to measure human IL-1 alpha in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

    The IL1 alpha ELISA set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    Recognizes both natural and recombinant human IL1a.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformReagents

Properties

  • RelevanceIL1 is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
  • Alternative names
    • Hematopoietin 1
    • IL 1 alpha
    • IL1A
    • IL1F1
    • Interleukin 1 alpha
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab47350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL1 alpha ELISA Set (without plates) images

References for Human IL1 alpha ELISA Set (without plates) (ab47350)

ab47350 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"