Human IL1 beta ELISA Set (without plates) (ab47351)

Overview

  • Product nameHuman IL1 beta ELISA Set (without plates)
  • Tests
    5 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    15.6 pg/ml - 500 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-1β Human in vitro ELISA Set is designed for the quantitative measurement of IL-1β in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    This ELISA Set is intended to measure human IL-1b in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

    The IL-1b ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    Recognizes both natural and recombinant human IL1b.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformReagents

Properties

  • RelevanceInterleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.
  • Cellular localizationSecreted; the lack of a specific hydrophobic segment in the precursor sequence suggests that IL1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Alternative names
    • Catabolin
    • IL1 BETA
    • IL1B
    • IL1F2
    • interleukin 1, beta
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab47351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
  • Application notesELISA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Human IL1 beta ELISA Set (without plates) images

    • IL1 beta Standard curve ranging from 15.6 to 500 pg/ml

    References for Human IL1 beta ELISA Set (without plates) (ab47351)

    ab47351 has not yet been referenced specifically in any publications.

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    The antibodies of ab47351 are both monoclonals, raised in mice.



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