Overview

  • Product name
    Human IL12p70 ELISA Set (without plates)
    See all IL12p70 kits
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 3 pg/ml
  • Range
    200 pg/ml - 6.25 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-12 Human in vitro ELISA Set is designed for the quantitative measurement of IL-12 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    The IL12p70 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    This ELISA Set is intended to measure human IL12p70 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

    Sensitivity: < 3 pg / ml

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Reagents

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests
    Detection biotinylated anti-IL-12 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml
    IL-12 Capture antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml
    IL12p70 Standard (Lyophilized) Yellow 5 vials 10 vials 15 vials
    Ready-to-use TMB 2 vials 4 vials 6 vials
    Streptavidin-HRP 1 vial 2 vials 3 vials
  • Research areas
  • Relevance
    Interleukin 12 is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 35 kD cytokine receptor like subunit encoded by IL12A gene, and a 40 kD subunit encoded by IL12B. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. It is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.
  • Alternative names
    • CLMF p35
    • CLMF p40
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit
    • Cytotoxic lymphocyte maturation factor 40 kDa subunit
    • IL 12A
    • IL 12B
    • NK cell stimulatory factor chain 1
    • NK cell stimulatory factor chain 2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab48488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Images

    • IL-12 Standard curve ranging from 6.25 to 200 pg/mL

    References

    ab48488 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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