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|Sample type||Average %||Range|
|Cell culture extracts||93.45||93% - 103%|
|Tissue Extracts||91.92||82% - 102%|
Abcam’s Human IL1RA ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL1RA in cell lysates and tissue lysates.
This assay employs an antibody specific for Human IL1RA coated on a 96-well plate. Standards and samples are pipetted into the wells and IL1RA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL1RA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL1RA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|2x Cell lysate buffer||1 x 5ml|
|300X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent||1 x 15ml|
|5X Sample Diluent Buffer||1 x 10ml|
|Biotinylated anti-Human IL-1RA||2 vials|
|IL-1RA Microplate (12 x 8 wells)||1 unit|
|Recombinant Human IL-1RA Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
IL1RA measured in RIPA lysates from control cells (-) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours (IL-1Ra per million cells; duplicates +/- SD). 5e6 cells were lysed in 0.1 mL, and tested in the dilution range of 1/1-1/40.
IL1RA measured in culture supernatants from control (-) or activated PBMCs ((PHA) 48 hours with 2% PHA-M; LifeTechnologies) and in human biological fluids. Signals from plasma and serum were below level of detection (duplicates +/- SD).
Standard curve with background signal subtracted (duplicates; +/- SD).
Representative standard curve using ab100565
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"