Human IL6 ELISA Set (without plates) (ab48478)

Overview

  • Product nameHuman IL6 ELISA Set (without plates)
    See all IL6 kits
  • Tests
    5 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    200 pg/ml - 6.25 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-6 Human in vitro ELISA Set is designed for the quantitative measurement of IL-6 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    The IL6 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    This ELISA set is intended to measure human IL6 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.


    Sensitivity: < 2 pg / ml

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformReagents

Properties

  • FunctionCytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in diseaseGenetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • B-cell stimulatory factor 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Hepatocyte stimulating factor
    • HSF
    • Hybridoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL-6
    • IL6
    • IL6_HUMAN
    • Interferon beta-2
    • Interleukin 6
    • Interleukin-6
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab48478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
  • Application notesELISA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Human IL6 ELISA Set (without plates) images

    • IL-6 Standard curve ranging from 6.25 to 200 pg/mL

    References for Human IL6 ELISA Set (without plates) (ab48478)

    ab48478 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. I apologise for the delay in obtaining this information for you. We have been waiting for confirmation from our source.

    I can confirm the reagents and materials required but not provided with ab48478 IL6 Human Eli...

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    Thank you for contacting us. I apologise for the delay.
    Unfortunately any of these kits have been tested with human ascites. However, they should work as they are suitable for all body fluids.
    The only problem I would suspect is the high conc...

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    Thank you for contacting us.

    1) Our Elipair kits only contain the reagents needed to perform the assay, i.e. they include both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) an...

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    Thank you for your reply.

    I can suggest the same would apply for ab48478. I can recommend to purchase ELISA plates specifically. to ensure they are of the higher quality required.

    I hope this will be helpful. If you have any further...

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    Thank you for contacting us. Overnight Incubation of plate at 4C is sort of general recommendation we provide to our customers. 3-4 hours at 4C can also be tried; the assay should be OK However in case of no readings we would be suggesting the over...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"