Overview

  • Product name
    Human Immunoglobulin E ELISA Kit (IgE)
    See all IgE kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Serum 16 <=7.2%
    Inter-assay
    Sample n Mean SD CV%
    Serum 16 <=7.6%
  • Sample type
    Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.27 IU/ml
  • Range
    0 IU/ml - 200 IU/ml
  • Assay duration
    Multiple steps standard assay
  • Product overview

    Abcam’s Immunogobulin E (IgE) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgE in serum and plasma.


    The essential reagents required for an immunoenzymometric assay include high affinity and specificity antibodies. In this procedure, the immobilization takes place during the assay at the surface of a microplate well through the interaction of streptavidin coated on the well and exogenously added biotinylated monoclonal anti-IgE antibody. Upon mixing monoclonal biotinylated antibody, and a serum containing the native antigen, reaction results between the native antigen and the antibody,forming an Antibody-Antigen complex. Simultaneously, the complex is deposited to the well through the high affinity reaction of streptavidin and biotinylated antibody. After a suitable incubation period, the antibody-antigen bound fraction is separated from unbound antigen by decantation or aspiration. Another antibody (directed at a different epitope) labelled with an enzyme is added. Another interaction occurs to form an Enzyme labelled Antibody-Antigen-Biotinylated-Antibody complex on the surface of the wells. Excess enzyme is washed off via a wash step. A suitable substrate is added to produce colour measurable with the use of a microplate spectrophotometer. The enzyme activity on the well is directly proportional to the native free antigen concentration. By utilizing several different serum references of known antigen concentration, a dose response curve can be generated from which the antigen concentration of an unknown can be ascertained.


    Get higher sensitivity in only 90 minutes with Human IgE ELISA Kit (ab195216) from our SimpleStep ELISA® range.


     

  • Notes

    IgE is an antibody isotype, found only in mammals. Although IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are ~150 ng/mL, compared to 10 mg/mL for the IgGs (the isotypes responsible for most of the classical adaptive immune response) - it is capable of triggering the most powerful immune reactions. Most of our knowledge of IgE has come from an allergy known as type 1 hypersensitivity.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    50X Washing Solution 1 x 20ml
    Cover Foil 1 unit
    IgE Biotin Conjugate 1 x 13ml
    IgE HRP Conjugate 1 x 13ml
    IgE Standard 0 - 0 mU/mL 1 x 1ml
    IgE Standard 1 - 5 mU/mL 1 x 1ml
    IgE Standard 2 - 25 mU/mL 1 x 1ml
    IgE Standard 3 - 50 mU/mL 1 x 1ml
    IgE Standard 4 - 150 mU/mL 1 x 1ml
    IgE Standard 5 - 400 mU/mL 1 x 1ml
    Stop Solution 1 x 15ml
    Streptavidin Coated Microplate (12 x 8 wells) 1 unit
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • Research areas
  • Relevance
    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localization
    Secreted
  • Alternative names
    • Ig epsilon chain C region
    • IgE
    • IGHE
    • immunoglobulin heavy constant epsilon

Associated products

Applications

Our Abpromise guarantee covers the use of ab178659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

Protocols

References

ab178659 has not yet been referenced specifically in any publications.

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