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Abcam’s Immunogobulin E (IgE) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgE in serum and plasma.
The essential reagents required for an immunoenzymometric assay include high affinity and specificity antibodies. In this procedure, the immobilization takes place during the assay at the surface of a microplate well through the interaction of streptavidin coated on the well and exogenously added biotinylated monoclonal anti-IgE antibody. Upon mixing monoclonal biotinylated antibody, and a serum containing the native antigen, reaction results between the native antigen and the antibody,forming an Antibody-Antigen complex. Simultaneously, the complex is deposited to the well through the high affinity reaction of streptavidin and biotinylated antibody. After a suitable incubation period, the antibody-antigen bound fraction is separated from unbound antigen by decantation or aspiration. Another antibody (directed at a different epitope) labelled with an enzyme is added. Another interaction occurs to form an Enzyme labelled Antibody-Antigen-Biotinylated-Antibody complex on the surface of the wells. Excess enzyme is washed off via a wash step. A suitable substrate is added to produce colour measurable with the use of a microplate spectrophotometer. The enzyme activity on the well is directly proportional to the native free antigen concentration. By utilizing several different serum references of known antigen concentration, a dose response curve can be generated from which the antigen concentration of an unknown can be ascertained.
Get higher sensitivity in only 90 minutes with Human IgE ELISA Kit (ab195216) from our SimpleStep ELISA® range.
IgE is an antibody isotype, found only in mammals. Although IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are ~150 ng/mL, compared to 10 mg/mL for the IgGs (the isotypes responsible for most of the classical adaptive immune response) - it is capable of triggering the most powerful immune reactions. Most of our knowledge of IgE has come from an allergy known as type 1 hypersensitivity.
|Components||1 x 96 tests|
|50X Washing Solution||1 x 20ml|
|Cover Foil||1 unit|
|IgE Biotin Conjugate||1 x 13ml|
|IgE HRP Conjugate||1 x 13ml|
|IgE Standard 0 - 0 mU/mL||1 x 1ml|
|IgE Standard 1 - 5 mU/mL||1 x 1ml|
|IgE Standard 2 - 25 mU/mL||1 x 1ml|
|IgE Standard 3 - 50 mU/mL||1 x 1ml|
|IgE Standard 4 - 150 mU/mL||1 x 1ml|
|IgE Standard 5 - 400 mU/mL||1 x 1ml|
|Stop Solution||1 x 15ml|
|Streptavidin Coated Microplate (12 x 8 wells)||1 unit|
|Strip holder||1 unit|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab178659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab178659 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"