Human Insulin degrading enzyme / IDE peptide (ab32215)

Overview

Description

  • NatureSynthetic

Specifications

Our Abpromise guarantee covers the use of ab32215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Insulin degrading enzyme / IDE antibody (ab32216)

  • FormLiquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • Abeta-degrading protease
    • FLJ35968
    • Ide
    • IDE_HUMAN
    • Insulin protease
    • Insulin-degrading enzyme
    • Insulinase
    • Insulysin
    • OTTHUMP00000020097
    see all
  • FunctionPlays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
  • Sequence similaritiesBelongs to the peptidase M16 family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localizationCytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
  • Information by UniProt

References for Human Insulin degrading enzyme / IDE peptide (ab32215)

ab32215 has not yet been referenced specifically in any publications.

Product Wall

This peptide (ab32215) can be used with studies using http://www.abcam.com/ab32216.html as blocking peptide to check any potencial non-specific signal. It has not been tested in any functional and activity assays.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"