Abcam’s Insulin like Growth Factor 1 (IGF1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Insulin like Growth Factor 1 concentrations in Human cell culture supernatants, serum and plasma.
An Insulin like Growth Factor 1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Insulin like Growth Factor 1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Insulin like Growth Factor 1 captured in plate.
Get results in 90 minutes with Human IGF1 ELISA Kit (ab211651) from our SimpleStep ELISA® range.
|Components||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent N Concentrate||1 x 30ml|
|1X Plasma/Serum Pretreatment Buffer||1 x 7.5ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|70X Biotinylated Human Insulin like Growth Factor 1 Antibody||1 x 90µl|
|Chromogen Substrate||1 x 8ml|
|Insulin like Growth Factor 1 Microplate (12 x 8 well strips)||1 unit|
|Insulin like Growth Factor 1 Standard||1 vial|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab108873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
Representative Standard Curve using ab108873.
ab108873 has not yet been referenced specifically in any publications.