Human Interferon gamma ELISA Kit (IFNG)

(ab100537)

Overview

  • Product name

    Human Interferon gamma ELISA Kit (IFNG)


    See all Interferon gamma kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    20.6 pg/ml - 15000 pg/ml
  • Recovery

    > 87 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.53 84% - 104%
    Plasma 86.82 81% - 102%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Interferon gamma (IFNG) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Interferon gamma- in plasma, and cell culture supernatants. (Human Interferon gamma concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for Human Interferon gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and Interferon gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Interferon gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Interferon gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Human Interferon gamma in normal serum with ab100537, only in serum spiked with Human Interferon gamma.


    Get results in 90 minutes with Human IFN gamma ELISA Kit (ab174443) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human Interferon gamma 2 vials
    Interferon gamma Microplate (12 x 8 wells) 1 unit
    Recombinant Human Interferon gamma Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
  • Alternative names
    • IFG
    • IFI
    • IFN gamma
    • IFN Immune
    • IFNG
    • Interferon gamma
    • Interferon Gamma Precursor
    • Macrophage Activating Factor
    • MAF
    • T Cell Interferon
    • Type II Interferon
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab100537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100537

  • Representative standard curve using ab100537

Protocols

References

ab100537 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your patience. I have checked on the bovine IFN-gamma ELISA Kit. Unfortunately, the kit currently is not available in our catalog as the quality control tests have not yet been completed.  In general, due to the low homology between...

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Thank you for contacting us.   We have the following products in our catalog: ab108863: Interferon gamma Human ELISA Kit We have tested the cross-reactivity with bovine samples and it is very low, around 1 %. We have never tested samples from gu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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