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|Sample type||Average %||Range|
|Cell culture extracts||92.25||82% - 103%|
|Tissue Extracts||91.73||80% - 101%|
Abcam’s Interferon gamma (IFNG) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Interferon gamma in cell lysates and tissue lysates.
This assay employs an antibody specific for Human Interferon gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and Interferon gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Interferon gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Interferon gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|2X Cell Lysate Buffer||1 x 5ml|
|400X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent||1 x 15ml|
|5X Sample Diluent Buffer||1 x 10ml|
|Biotinylated anti-Human Interferon gamma||2 vials|
|Interferon gamma Microplate (12 x 8 wells)||1 unit|
|Recombinant Human Interferon gamma Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100538 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"