Human Interferon gamma ELISA Kit (IFNG) (ab108863)


  • Product nameHuman Interferon gamma ELISA Kit (IFNG)
    See all Interferon gamma kits
  • Detection methodColorimetric
  • Precision
    Sample n Mean SD CV%
    Overall 3%
    Sample n Mean SD CV%
    Overall 8%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    > 0.01 ng/ml
  • Range
    0.016 ng/ml - 1 ng/ml
  • Recovery

    97 %

  • Assay time
    5h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Interferon gamma (IFNG) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Interferon gamma levels in cell culture supernatants, serum and plasma.

    An Interferon gamma specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Interferon gamma specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Interferon gamma captured in plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • FunctionProduced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificityReleased primarily from activated T lymphocytes.
  • Involvement in diseaseIn Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similaritiesBelongs to the type II (or gamma) interferon family.
  • Post-translational
    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • IFN-gamma
    • IFNG
    • Immune interferon
    • Interferon gamma
    see all
  • Database links


Our Abpromise guarantee covers the use of ab108863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Human Interferon gamma ELISA Kit (IFNG) images

  • Representative Standard Curve using ab108863.


References for Human Interferon gamma ELISA Kit (IFNG) (ab108863)

ab108863 has not yet been referenced specifically in any publications.

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Thank you for your patience. I have checked on the bovine IFN-gamma ELISA Kit. Unfortunately, the kit currently is not available in our catalog as the quality control tests have not yet been completed.  In general, due to the low homology between...

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Thank you for contacting us.   We have the following products in our catalog: ab108863: Interferon gamma Human ELISA Kit We have tested the cross-reactivity with bovine samples and it is very low, around 1 %. We have never tested samples from gu...

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