Human Interferon Gamma + IL2 ELISPOT Kit (ab48448)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameHuman Interferon Gamma + IL2 ELISPOT Kit
See all Interferon Gamma + IL2 kits -
Detection methodColorimetric
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Sample typeCell culture supernatant, Cell culture extracts
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Assay typeSandwich (qualitative)
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Assay durationMultiple steps standard assay
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Species reactivityReacts with: Human
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Product overview
The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, oncology, infectious diseases, autoimmune diseases and transplantation.
This Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
The dual colour Elispot allows you to monitor the production of two cytokines simultaneously in the same well.
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Tested applicationsSuitable for: ELISpotmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 5 x 96 tests 10 x 96 tests 10 x concentrate buffer for the preparation of AEC buffer 1 x 5ml 2 x 5ml 50 x concentrate AEC substrate buffer 1 x 1ml 2 x 1ml 96 PVDF-bottomed-well plates. 5 units 10 units Anti-FITC antibody HRP conjugate 1 x 120µl 2 x 100µl Bovine Serum Albumin 1 x 1g 2 x 1g Human IFN? Capture antibody 1 x 500µl 2 x 500µl Human IL-2 Capture antibody 1 x 500µl 2 x 500µl IFNγ FITC conjugated detection antibody 1 vial 2 vials IL-2 Biotinylated detection antibody 1 vial 2 vials Ready-to-use BCIP/NBT substrate buffer 2 x 27ml 2 x 50ml Streptavidin - Alkaline Phosphatase conjugated 1 x 50µl 2 x 50µl -
Research areas
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RelevanceIFN-gamma is produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
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Cellular localizationSecreted
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Alternative names
- IFN gamma
- IFNG
- IL2
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Database links
- Entrez Gene: 3458 Human
- Entrez Gene: 3558 Human
- SwissProt: P01579 Human
- SwissProt: P60568 Human
Applications
Our Abpromise guarantee covers the use of ab48448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISpot | Use at an assay dependent dilution. |
References
ab48448 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"