Abcam’s Interferon omega Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Interferon omega concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Human Interferon omega specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Human Interferon omega detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Human Interferon omega captured on the plate.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||4 units|
|Biotin-Conjugate anti-Interferon omega monoclonal antibody||1 x 200µl|
|Interferon omega Standard lyophilized (600 pg/mL upon reconstitution)||2 x 50µl|
|Microplate coated with monoclonal antibody to Interferon omega (12 x 8 wells)||1 unit|
|Sample Diluent||1 x 12ml|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|Streptavidin-HRP||1 x 150µl|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119529 has not yet been referenced specifically in any publications.