Human Interleukin-5 R alpha ELISA Kit (IL-5 Ra) (ab155435)

Overview

  • Product name
    Human Interleukin-5 R alpha ELISA Kit (IL-5 Ra)
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 0.15 ng/ml
  • Range
    0.164 ng/ml - 40 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 114 95% - 122%
    Serum 86.65 70% - 97%
    Plasma 78.97 69% - 86%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Interleukin-5 R alpha (IL-5 Ra) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human Interleukin-5 R alpha (IL-5 Ra) in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human Interleukin-5 R alpha (IL-5 Ra) coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-5R alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-5 R alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 R alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab155435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab155435 -  Assay Diluent B.

  • Representative standard curve using ab155435 - Assay Diluent C.

     

Protocols

References

ab155435 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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