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Product Name
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human intestinal Alkaline Phosphatase antibody (Biotin)
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See all Human intestinal alkaline phosphatase antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to human intestinal Alkaline Phosphatase (Biotin)
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Immunogen
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Alkaline Phosphatase [human intestine]
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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Dot, ELISA, IHC-Fr, IM, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Immunoblotting (western or dot blot)
ELISA (1:4,000 to 1:20,000)
Immunohistochemistry
Conjugation
as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
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Cellular localization
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Cell Membrane
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Research areas
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Signal Transduction >> Metabolism >> Energy Metabolism
Immunology >> Immune System Diseases >> Autoimmune
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Relevance
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The intestinal isozyme of alkaline phosphatase is one of the isozymes of alkaline phosphatase found in the serum of healthy subjects. It is located in the intestinal brush-border.
Alkaline phosphatases are non-specific phosphomonoesterases that catalyze a hydrolysis reaction, via a phosphoseryl intermediate, to produce inorganic phosphate and the corresponding alcohol, optimally at high pH.
This polyclonal antibody reacts with alkaline phosphatase from human intestine showing a 60 kD band against this enzyme under reducing conditions of SDS-PAGE.
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human intestinal Alkaline Phosphatase antibody (Biotin) (ab7323) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Conjugation notes
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Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
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Storage buffer
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0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS
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Purification notes
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This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
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Form
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Liquid
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Conjugation
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Biotin
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Concentration
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10.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this human intestinal Alkaline Phosphatase antibody (Biotin) is on this datasheet. But please do contact us if you would like any reassurance! |
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References for human intestinal Alkaline Phosphatase antibody (Biotin) (ab7323)
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This product has been used in:
Krohn J & Rodahl E Expression of 5'-nucleotidase and alkaline phosphatase in human aqueous drainage channels. Acta Ophthalmol Scand 80:642-51 (2002). PubMed: 12485287
If you publish research using ab7323 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Human intestinal alkaline phosphatase
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human intestinal Alkaline Phosphatase antibody (Biotin) - more information
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