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Product Name
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human intestinal Alkaline Phosphatase antibody (HRP)
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See all Human intestinal alkaline phosphatase antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to human intestinal Alkaline Phosphatase (HRP)
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Immunogen
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Alkaline Phosphatase [human intestine]
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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Dot, ELISA, IHC-Fr, IM, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
For ELISA use at 1:1,000 to 1:5,000
Useful for peroxidase-antibody based enzymatic assays.
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Cellular localization
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Cell Membrane
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Research areas
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Signal Transduction >> Metabolism >> Energy Metabolism
Immunology >> Immune System Diseases >> Autoimmune
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Relevance
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The intestinal isozyme of alkaline phosphatase is one of the isozymes of alkaline phosphatase found in the serum of healthy subjects. It is located in the intestinal brush-border.
Alkaline phosphatases are non-specific phosphomonoesterases that catalyze a hydrolysis reaction, via a phosphoseryl intermediate, to produce inorganic phosphate and the corresponding alcohol, optimally at high pH.
This polyclonal antibody reacts with alkaline phosphatase from human intestine showing a 60 kD band against this enzyme under reducing conditions of SDS-PAGE.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Conjugation notes
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Horseradish Peroxidase (HRP)
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Storage buffer
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0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS
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Purification notes
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This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
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Form
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Liquid
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Conjugation
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Horse Radish Peroxidase
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Concentration
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10.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this human intestinal Alkaline Phosphatase antibody (HRP) is on this datasheet. But please do contact us if you would like any reassurance! |
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References for human intestinal Alkaline Phosphatase antibody (HRP) (ab7324)
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This product has been used in:
Giatromanolaki A et al. Down-regulation of intestinal-type alkaline phosphatase in the tumor vasculature and stroma provides a strong basis for explaining amifostine selectivity. Semin Oncol 29:14-21 (2002). PubMed: 12577238
If you publish research using ab7324 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Human intestinal alkaline phosphatase
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