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Anti-human intestinal Alkaline Phosphatase antibody (HRP) (ab7324)

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Overview

Product name

Anti-human intestinal Alkaline Phosphatase antibody (HRP)
See all Human intestinal alkaline phosphatase products (10) ...

Description

Rabbit polyclonal to human intestinal Alkaline Phosphatase (HRP)

Conjugation

HRP

Conjugation notes

Horseradish Peroxidase (HRP)

Tested applications

Dot Blot, ELISA, IHC-Fr, Immunomicroscopy, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Alkaline Phosphatase [human intestine]

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.

Primary antibody notes

This polyclonal antibody reacts with alkaline phosphatase from human intestine showing a 60 kD band against this enzyme under reducing conditions of SDS-PAGE.

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Metabolism >> Energy Metabolism
Immunology >> Immune System Diseases >> Autoimmune

Applications

Show applications key

Our Abpromise guarantee covers the use of ab7324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Dot Blot

     

  • ELISA

     

  • IHC-Fr

     

  • Immunomicroscopy

     

  • WB

     

Application notes

For ELISA use at 1:1,000 to 1:5,000
Useful for peroxidase-antibody based enzymatic assays.

Target

Sequence similarities

Belongs to the alkaline phosphatase family.

Cellular localization

Cell membrane.

Target information above from: UniProt accessionP09923 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Alkaline phosphatase intestinal antibody
  • Alkaline phosphomonoesterase antibody
  • ALPI antibody
  • Glycerophosphatase antibody
  • IAP antibody
  • Intestinal alkaline phosphatase antibody
  • Intestinal-type alkaline phosphatase antibody
  • Kasahara isozyme antibody
  • OTTHUMP00000164357 antibody
  • PPBI_HUMAN antibody
see all

Database links

References for Anti-human intestinal Alkaline Phosphatase antibody (HRP) (ab7324)

This product has been referenced in:

  • Giatromanolaki Aet al. Down-regulation of intestinal-type alkaline phosphatase in the tumor vasculature and stroma provides a strong basis for explaining amifostine selectivity. Semin Oncol 29:14-21 (2002).Read more (PubMed: 12577238) »

See 1 publication for this product

Publishing research using ab7324? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"