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Products:Immunology >> Immune System Diseases >> Autoimmune
Overview
Product name
Anti-Human intestinal alkaline phosphatase antibody
See all Human intestinal alkaline phosphatase products (10) ...
Description
Rabbit polyclonal to Human intestinal alkaline phosphatase
Specificity
The alkaline phosphatase sequence in different tissue types is significantly homologous so this antibody may cross react.
Tested applications
Conjugation, Dot Blot, ELISA, IHC-Fr, IP, WB, IHC-Pmore details
Cross reactivity
Reacts with
Rabbit, Human
Immunogen
Alkaline Phosphatase [human intestine]
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Primary antibody notes
This polyclonal antibody reacts with alkaline phosphatase from human intestine showing a 60 kD band against this enzyme under reducing conditions of SDS-PAGE.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Energy Metabolism
Immunology >> Immune System Diseases >> Autoimmune
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Conjugation
Dot Blot
ELISA
IHC-Fr
IP
- 1 Image
IHC-P
Application notes
Conjugation: Use at an assay dependent dilution.
Dot: Use at an assay dependent dilution.
ELISA: 1/20000 - 1/100000.
IHC-P: 1/500 (PMID 19264912).
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/10000. Detects a band of approximately 60 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Sequence similarities
Belongs to the alkaline phosphatase family.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP09923
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alkaline phosphatase intestinal antibody
- Alkaline phosphomonoesterase antibody
- ALPI antibody
see all
Database links
- Entrez Gene: 248 Human
- Omim: 171740 Human
- SwissProt: P09923 Human
- Unigene: 284255 Human
- Unigene: 37009 Human
Anti-Human intestinal alkaline phosphatase antibody images:
Western blot - human intestinal Alkaline Phosphatase antibody (ab7322)
Anti-Human intestinal alkaline phosphatase antibody (ab7322) at 1/10000 dilution + Alkaline
Phosphatase (Human Intestine)
Secondary
HRP Goat-a-Rabbit IgG [H&L]
at 1/4000 dilution
Performed under reducing conditions.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Human intestinal alkaline phosphatase antibody (ab7322)
ab7322 (2µg/ml) staining human intestinal alkaline phosphatase in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of mucosal epithelium.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-Human intestinal alkaline phosphatase antibody (ab7322)
This product has been referenced in:
- Campbell ELet al. Resolvin E1-induced intestinal alkaline phosphatase promotes resolution of inflammation through LPS detoxification. Proc Natl Acad Sci U S A 107:14298-303 (2010).Read more (PubMed: 20660763) »
- Asai-Coakwell Met al. Incomplete penetrance and phenotypic variability characterize Gdf6-attributable oculo-skeletal phenotypes. Hum Mol Genet 18:1110-21 (2009). WB; Human.Read more (PubMed: 19129173) »
See all 7 publications for this product
Publishing research using ab7322? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"