Human intestinal alkaline phosphatase antibody (ab7322)

Product Name 

Human intestinal alkaline phosphatase antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Human intestinal alkaline phosphatase

Immunogen 

Alkaline Phosphatase [human intestine]

Reacts with 
(species key)

Hu, Rb

Tested applications 
(see key)

Conjugation, Dot, ELISA, IHC-Fr, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Conjugation: Use at an assay dependent dilution.
Dot: Use at an assay dependent dilution.
ELISA: 1/20000 - 1/100000.
IHC-P: 1/500 (PMID 19264912).
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/10000. Detects a band of approximately 60 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell Membrane

Research areas 

Signal Transduction >> Metabolism >> Energy Metabolism
Immunology >> Immune System Diseases >> Autoimmune

Relevance 

The intestinal isozyme of alkaline phosphatase is one of the isozymes of alkaline phosphatase found in the serum of healthy subjects. It is located in the intestinal brush-border.
Alkaline phosphatases are non-specific phosphomonoesterases that catalyze a hydrolysis reaction, via a phosphoseryl intermediate, to produce inorganic phosphate and the corresponding alcohol, optimally at high pH.

This polyclonal antibody reacts with alkaline phosphatase from human intestine showing a 60 kD band against this enzyme under reducing conditions of SDS-PAGE.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA

Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS

Purification notes 

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.

Form 

Liquid

Concentration 

10.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Human intestinal alkaline phosphatase antibody images, references, products related to ab7322 and other tools.

Human intestinal alkaline phosphatase antibody images:

References for Human intestinal alkaline phosphatase antibody (ab7322)

  This product has been used in: (two most recent references)

Jedlicka P et al. Ets transcription factors control epithelial maturation and transit and crypt-villus morphogenesis in the mammalian intestine. Am J Pathol 174:1280-90 (2009). IHC-P. PubMed: 19264912

Kirkland SC Type I collagen inhibits differentiation and promotes a stem cell-like phenotype in human colorectal carcinoma cells. Br J Cancer 101:320-6 (2009). ELISA; Human. PubMed: 19568234

See all 5 publication references for this product.


If you publish research using ab7322 please let us know so that we can cite the reference on this datasheet.

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