Anti-Human intestinal alkaline phosphatase antibody (ab97532)

Overview

  • Product nameAnti-Human intestinal alkaline phosphatase antibody
    See all intestinal alkaline phosphatase primary antibodies
  • Description
    Rabbit polyclonal to Human intestinal alkaline phosphatase
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 3 and 216 of Human intestinal alkaline phosphatase (NP_001622)

  • Positive control
    • 293T, A431, H1299, HeLa, Molt-4 and Raji cell lysate. HepG2 whole cell lysate. Paraffin-embedded SG xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab97532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/500.

Target

Anti-Human intestinal alkaline phosphatase antibody images

  • Anti-Human intestinal alkaline phosphatase antibody (ab97532) at 1/1000 dilution + Hep G2 whole cell lsyate at 30 µg

    Predicted band size : 57 kDa
  • Immunohistochemical analysis of paraffin-embedded SG xenograft, using ab97532 at 1/100 dilution.

References for Anti-Human intestinal alkaline phosphatase antibody (ab97532)

This product has been referenced in:
  • Shin J  et al. The intestinal epithelial cell differentiation marker intestinal alkaline phosphatase (ALPi) is selectively induced by histone deacetylase inhibitors (HDACi) in colon cancer cells in a Kruppel-like factor 5 (KLF5)-dependent manner. J Biol Chem 289:25306-16 (2014). WB ; Human . Read more (PubMed: 25037223) »

See 1 Publication for this product

Product Wall

Thank you for submitting an Abreview of ab97532. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in an untested species (rat), we would like to follow up on this....

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (small intestine)
Loading amount 25 µg
Specification small intestine
Gel Running Conditions Reduced Denaturing (8 % gel)
Blocking step 3% BSA, 5% Milk, Blocking Buffer for fluorescent Western Blotting as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

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Verified customer

Submitted May 12 2012

DISCOUNT CODE: xxx Ablaufdatum: dd.mm.2012 Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab97532 in der Ratte zu testen, annehmen möchten. Wie besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetze...

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Vielen Dank für Ihre Anfrage. Das Immunogen, das zur Herstellung von ab95462 verwendet wird, ist ein synthetisches Peptid, das von der Alkalischen Phosphatase der Ratte mit der SwissProt-ID P08289 abgeleitet ist. Leider weist dieses Peptid keine Seq...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"