Overview

  • Product nameHuman IP-10 ELISA Kit
    See all IP10 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 8 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 101.34 84% - 105%
    Serum 94.49 82% - 104%
    Plasma 96.13 83% - 103%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IP-10 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IP-10 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IP-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IP-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IP-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IP-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionChemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Interferon gamma induced factor MOB1, mouse, homolog of
    • Interferon gamma induced protein 10
    • 10 kDa interferon gamma induced protein
    • 10 kDa interferon gamma-induced protein
    • C X C motif chemokine 10
    • C7
    • Chemokine (C X C motif) ligand 10
    • Chemokine CXC motif ligand 10
    • Crg 2
    • CRG2
    • CXCL10
    • CXCL10(1-73)
    • CXL10_HUMAN
    • Gamma IP10
    • Gamma-IP10
    • gIP 10
    • GIP10
    • IFI10
    • INP 10
    • INP10
    • Interferon activated gene 10
    • Interferon gamma induced cell line
    • Interferon inducible cytokine IP 10
    • Interferon inducible cytokine IP10
    • IP 10
    • IP-10
    • Mob 1
    • MOB1
    • Protein 10 from interferon (gamma) induced cell line
    • SCYB10
    • Small inducible cytokine B10
    • Small inducible cytokine B10 precursor
    • Small inducible cytokine subfamily B (Cys X Cys) member 10
    • Small inducible cytokine subfamily B CXC member 10
    • Small inducible cytokine subfamily B, member 10
    • Small-inducible cytokine B10
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IP-10 ELISA Kit images

  • Standard curve in dilutent B with background signal subtracted (duplicates; +/- SD).

  • Standard curve in dilutent A with background signal subtracted (duplicates; +/- SD).

  • IP-10 measured in undiluted cell culture supernatants from control (-) or treated ((PHA) 2% PHA-M; LifeTechnologies) PBMCs or in supernatants from control THP-1 cells (-) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) with 1 ug x mL-1 of LPS (Sigma) for the last 6 hours (P/L) (duplicates +/- SD).

  • IP-10 measured in undiluted biological fluids (duplicates +/- SD). Signals from mouse and rat samples were below level of detection.

  • Representative Standard Curve using ab100578.

  • Representative Standard Curve using ab100578.

Protocols

References for Human IP-10 ELISA Kit (ab100579)

ab100579 has not yet been referenced specifically in any publications.

Product Wall

IP-10 Human ELISA

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Easy to use and easy to follow ELISA kit. Would recommend this kit for cell culture samples.
Username

Abcam user community

Verified customer

Submitted Jul 21 2015

CXCL10 secretion by macrophages

Excellent Excellent 5/5 (Ease of Use)
Abreviews
CXCL10 secretion my human primary macrophages in culture for 3 days.
Human monocytes isolated by negative selection.
CXCL10 secretion by the M1 polarized macrophages is observed.
The kit worked perfectly well, no dilution necessary, the assay was performed in 5.30hr.
Username

Abcam user community

Verified customer

Submitted Mar 12 2014

Thank you for contacting us.

We unfortunately do not have these kits available for human samples. I however have contacted the product manager and asked them if these products are in pipeline, I will get back to you once I get it confirmed. Read More

I have contacted the lab who confirmed that it is not recommended to load less than 100 ul of sample per well as this may affect the quality of the results. For the best results, the protocol should be followed exactly.

Thank you for your inquiry. Unfortunately, there is no cross reactivity data available testing cynomolgus with the Human IP-10 ELISA kit (ab100579). However, I would expect the homology between cynomolgus and human to be quite high so I think it wo...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"