Overview

  • Product name
    Human IP-10 ELISA Kit
    See all IP-10 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    PBMC media 9 5.1%
    Inter-assay
    Sample n Mean SD CV%
    PBMC media 3 11.1%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    1.4 pg/ml
  • Range
    1.4 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 103 102% - 105%
    Cell culture media 96 95% - 98%
    Heparin Plasma 86 82% - 88%
    EDTA Plasma 101 99% - 103%
    Citrate Plasma 96 93% - 98%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    IP-10 (CXCL10) human in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IP-10 (CXCL10) protein in human serum and plasma samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    C-X-C motif chemokine 10 (CXCL10 or IP-10) is a small 10.8kD protein that is secreted by several cell types in response to interferon-gamma (IFNg). These cell types include monocytes, endothelial cells and fibroblasts. Upon secretion, CXCL10 is cleaved into an 8.7kD biologically active protein to function in chemotaxis for T-cells, NK cells, monocytes/macrophages and dendritic cells. In addition, CXCL10 has antitumor activity through the inhibition of bone marrow colony formation and angiogenesis. CXCL10 elicits its effects by binding to the cell surface chemokine receptor 3 (CXCR3).

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab173194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Example IP-10/CXCL10 standard curve. Background-subtracted data values (mean +/- SD) are graphed. A new standard curve must be generated for each assay performed.

  • Titration of PBMC conditioned media (+/- PHA) within the working range of the assay. Background subtracted data from triplicate measurements are plotted.

  • Data shows specific secrection of IP-10 (CXCL10) by human macrophages differentiated in culture for 3 days in a dose response to M1 (MCSF + IFNg).

Protocols

References

ab173194 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

CXCL10 secretion by human macrophages

Excellent Excellent 5/5 (Ease of Use)
Abreviews
the kit is very sensitive and specific. data shows secrection in a dose response by M1 (MCSF+IFNg) human macrophages differentiated in culture for 3 days.

Regarding speed, it took me about 2hrs to run the assay, very much time saving considering a minimum of 5hrs for the standard ELISA. Money wise, price is more or less the same as a standard ELISA.
Something interesting is the volume required is 50µl versus the 100µl of a standard ELISA.
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Verified customer

Submitted Jan 20 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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